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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2212-2218
DOI: https://doi.org/10.20546/ijcmas.2017.611.262


Molecular Characterization of Tirupati Isolate of Pigeonpea Sterility Mosaic Virus
K. Sailaja1*, B.V. Bhaskara Reddy2, L. Prasanthi2 and P. Sarada Jayalakshmi Devi3
1Department of Plant Pathology, S. V. Agricultural College, Tirupati, Acharya N. G. Ranga Agricultural University, Andhra Pradesh, India
2Institute of Frontier Technology, Regional Agricultural Research Station, Tirupati, Acharya N. G. Ranga Agricultural University, Andhra Pradesh, India
3Acharya N. G. Ranga Agricultural University, Guntur, Andhra Pradesh, India
*Corresponding author
Abstract:

Nucleotide sequence analysis of partial replicase gene of Pigeonpea sterility mosaic virus-Tirupati isolate shown 96.9 % similarity with ICRISAT-PPSMV-I followed by PPSMV-I-Chevella (81.2%), PPSMV-II-ICRISAT (55.3%) and <51% with other Emaraviruses. Based on 322bp partial replicase sequence homology studies, the present isolate is closely related to PPSMV-I causing SMD at ICRISAT. PPSMV- TPT - isolate formed unique clade with ICRISAT-PPSMV-I isolate within cluster that consists of PPSMV I-Chevella isolate in phylogenetic tree constructed based on partial replicase gene sequences of PPSMV-I, PPSMV-II and other Emaravirus sequences downloaded from GenBank.


Keywords: PPSMV, EMARaV, FMV, SMD.
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How to cite this article:

Sailaja, K., B.V. Bhaskara Reddy, L. Prasanthi and Sarada Jayalakshmi Devi, P. 2017. Molecular Characterization of Tirupati Isolate of Pigeonpea Sterility Mosaic Virus.Int.J.Curr.Microbiol.App.Sci. 6(11): 2212-2218. doi: https://doi.org/10.20546/ijcmas.2017.611.262