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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2121-2136
DOI: https://doi.org/10.20546/ijcmas.2017.611.250


Isolation and Characterization of Tomato Leaf Phyllosphere Methylobacterium and their Effect on Plant Growth
M. Senthilkumar1* and R. Krishnamoorthy2
1Department of Agricultural Microbiology, Tamil Nadu Agricultural University,Coimbatore-641 003, Tamil Nadu, India
2Department of Agricultural Microbiology, Agricultural College and Research Institute,Madurai 625 104, Tamil Nadu Agricultural University, Tamil Nadu, India
*Corresponding author
Abstract:

Phyllosphere methylotrophic bacteria from tomato leaves were isolated using selective Ammonia Mineral Salt (AMS) medium. Totally thirty two epiphytic bacterial isolates were selected based on their colony morphology and distinct pigmentation. All the isolates tested were Gram negative, rod shaped and positive for urease, catalase and oxidase activities. The preliminary screening of five randomly selected strains under in vitro for plant growth promoting characteristics and evaluated for their beneficial effects on the early growth of tomato showed positive for nitrogen fixation, ACC deaminase, siderophore and phytohormone productions were chosen for further studies. An in vitro experiment was conducted with 4 selected Methylobacterium spp. to evaluate their effect on tomato seed germination and vigor index. Among the isolates tested, Mt-Tm-13 had significantly increased the seed germination percentage (100%) earliness in radical emergence and vigor index. Plant inoculation studies indicated that Methylobacterium spp. strains provided a significant beneficial effect to the plants. In pot culture experiment, foliar application of Methylobacterium spp. (1% v/v concentration) showed that the inoculated plants had significant plant growth, biomass as well as yield attributes. The present study suggests that the Methylobacterium isolates may be used as bio-inoculants for sustainable tomato cultivation.


Keywords: Methylobacterium sp., Tomato, Phyllosphere bacteria, Plant growth promotion.
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How to cite this article:

Senthilkumar, M. and Krishnamoorthy, R. 2017. Isolation and Characterization of Tomato Leaf Phyllosphere Methylobacterium and their Effect on Plant Growth.Int.J.Curr.Microbiol.App.Sci. 6(11): 2121-2136. doi: https://doi.org/10.20546/ijcmas.2017.611.250