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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2079-2086
DOI: https://doi.org/10.20546/ijcmas.2017.611.245


Hybrid Purity Testing of Chilli Hybrid (Pusa Jwala × Arka Lohit) through RAPD and ISSR Molecular Markers
Udachappa U. Pujar*, Shantappa Tirakannanavar, R.C. Jagadeesha and N. Sandhyarani
Department of Crop Improvement and Biotechnology, Kittur Rani Channamma College of Horticulture, Arabhavi-591310, Karnataka, India
*Corresponding author
Abstract:

A fast and accurate genetic purity test of Fl hybrid plants is essential for seed production and accelerating advanced breeding generations in breeding programmes. DNA technology has great potential for enhancing purity assessment of hybrids. Chilli F1 hybrid (Pusa Jwala × Arka Lohit) and its parents Arka Lohit (male) and Pusa Jwala (female) were analyzed for the development of markers, identification of hybrids and ascertaining genetic purity in Capsicum annuum L. through PCR based Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSRs) DNA Markers. Twenty seven RAPD primers of Operon series and twenty ISSR primers were used for analysis. RAPD primer, OPA 15 and OPD 16 were found to be heteroallelic for parents. OPA 15 identified male specific amplicons of about 1200bp and OPD 16 produced male specific amplicon of about 1100bp in the hybrid (Pusa Jwala × Arka Lohit). The other repeats of different base pairs were homoallelic for both the parents. All twenty ISSR primers used were found to be homoallelic for both the parents. This was due to the RAPD marker system producing a greater number of markers than the ISSR system. OPD 16 identified a female-specific amplicon of about 800bp in the hybrid (Pusa Jwala × Arka Lohit). Comparison of the two RAPD banding pattern of the parents with respective hybrid clearly identified genuine hybrid. Thus RAPD markers individually have their own merits in the identification of parents and their hybrids. However, a combination of two PCR based markers can be used for testing the genetic purity of chilli seeds or seedlings, which will be more reliable substitute for GOT and a tool for seed certification. This study suggested that efficiency of RAPD assay to evaluate successfully hybridity determination, which would be a valuable genomic tool for the chilli breeders.


Keywords: Chilli, Hybridity test, RAPD, ISSR and GOT.
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How to cite this article:

Udachappa U. Pujar, Shantappa Tirakannanavar, R.C. Jagadeesha and Sandhyarani, N. 2017. Hybrid Purity Testing of Chilli Hybrid (Pusa Jwala × Arka Lohit) through RAPD and ISSR Molecular Markers.Int.J.Curr.Microbiol.App.Sci. 6(11): 2079-2086. doi: https://doi.org/10.20546/ijcmas.2017.611.245