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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 2065-2078
DOI: https://doi.org/10.20546/ijcmas.2017.611.244


Functional Attributes of Psychrotolerant Rhizobacteria from Wheat (Triticum aestivum L.) Rhizoshpere
Aamni Singh1 and Veena Khanna2*
1Department of Microbiology, Punjab Agricultural University, Ludhiana – 141001, Punjab, India
2Department of Plant Breeding and Genetics, Punjab Agricultural University,Ludhiana - 141001 Punjab, India
*Corresponding author
Abstract:

Cold-tolerant PGPRs are known to improve plant growth and nutrient uptake by an array of mechanisms, under stress and nutrient imbalance conditions. On the basis of growth potential we selected 43 PGPRs from pool of 85 rhizobacterial isolates obtained from wheat rhizospheric soil collected from different wheat growing fields. Phosphate and zinc solubilization efficiency along with phytohormones and siderophore producing ability at 10 and 20°C was also assessed. Rhizobacterial isolates A-43, A-13, A-12, A-36, A-25, A-22 and A-34 was potent phosphate and zinc solubilizers. IAA production was estimated in the range from 2.12 to 43.24 μg/ml and 2.40 to 68.36 μg/ml in the presence of tryptophan at 10 and 20°C respectively. Maximum GA production was recorded with A-43, A-13, A-12 and A-25 at 10 and 20°C. Similarly highest siderophore production was shown by bacterial culture A-6 (250.4 µg/ml and 90.5 µg/ml) and A-43 (225.9 µg/ml and 80.6 µg/ml) at both temperatures. Also, isolates A-43, A-36, A-13 and A-12 were resistant to many antibiotics like ampicillin (25 g/disc), tetracycline (10 g/disc), streptomycin (10 g/disc) etc. These four potent rhizobacterial isolates improved germination, root and shoot length and fresh weight of wheat seedlings as compared to uninoculated control under axenic conditions.


Keywords: PGPR, Rhizobacteria, Wheat, Zinc solubilizers.

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How to cite this article:

Aamni Singh and Veena Khanna. 2017. Functional Attributes of Psychrotolerant Rhizobacteria from Wheat (Triticum aestivum L.) Rhizoshpere.Int.J.Curr.Microbiol.App.Sci. 6(11): 2065-2078. doi: https://doi.org/10.20546/ijcmas.2017.611.244
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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