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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 963-970
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.101


Studies on the Multidrug Resistance to Pseudomonas aeruginosa Isolated from Infected Wounds
N.A. AL-, A.N. AL-Thwaini2, W.A. AL-Turk3 and K.I. ALtaif 3*
1AL-Imamein AL-Kadhimein Medical city, Baghdad, Iraq
2Genetic Engineering and Biotechnology institute for postgraduate studies,University of Baghdad, Iraq
3Faculty of pharmacy, Middle East University, Amman-Jordan
*Corresponding author
Abstract:

The infection wounds remain challenging and present a major global problem leading to many complications. All collected samples, 150 swab from patient’s wounds and hospital units were cultured on different media and tested biochemically. The profile of bacteria in the cultured samples revealed that, Staphylococcus aureus was the most common bacteria and represented in 23 isolates (18%), followed by Pseudomonas aeruginosa with 11 isolates ( 12%). The results showed that, all isolates were completely (100%) resistant to Ceftrixone, Cefepime, Ampicillin–Sulbactam, and Ceftazidime. Psedomonas aeruginosa showed the highest resistance to both Gentamycin, Trimethoprime–Sulfamethoxazole reached to 66.6% and to less extent against Aztreonam (61.1%). Polymerase Chain Reaction (PCR) was used to detect some of Metallo Beta Lactemase MBL genes responsible for antibiotic's resistance in P. aeruginosa isolates as, blaVIM and bla IMP. The results showed that six isolates of P. aeruginosa carried bla VIM, two had bla IMP.


Keywords: Pseudomonas, Resistance, Wounds,PCR,MBL.
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How to cite this article:

AL-Kadhmi, N.A., A.N. AL-Thwaini, W.A. AL-Turk and ALtaif, K.I. 2016. Studies on the Multidrug Resistance to Pseudomonas aeruginosa Isolated from Infected Wounds.Int.J.Curr.Microbiol.App.Sci. 5(5): 963-970. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.101