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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1829-1834
DOI: https://doi.org/10.20546/ijcmas.2017.611.218


In–Vitro Regeneration of Aloe Vera (Aloe barbadensis Mill)
Shashi Kiran1, Alice Tirkey1*, Zenu Jha2 and S.S. Porte3
1Department of Genetics and Plant Breeding, Indira Gandhi Krishi Vishwavidyalaya,College of Agriculture, Raipur- 492012, Chhattisgarh, India
2Department of Plant Molecular Biology and Biotechnology, Indira Gandhi Krishi Vishwavidyalaya, College of Agriculture, Raipur- 492012, Chhattisgarh, India
3Department of Soil Science, Indira Gandhi Krishi Vishwavidyalaya, College of Agriculture, Raipur- 492012, Chhattisgarh, India
*Corresponding author
Abstract:

The research was conducted in commercial tissue culture laboratory, IGKV, Raipur, C.G. from the period of March 2015 to August 2017. Present investigation was conducted to assess the suitable explant for callusing using different treatment of auxin and cytokinin (NAA and BAP) in semi-solid Murashige and Skoog (MS) media. The results revealed that the meristem shoot found to be best for callusing. Among five different treatments MS medium with 2 mg/l BAP + 2mg/l NAA was found best for multiple shoot initiation for meristem shoot. For root induction MS medium with 1.0 mg/1 IBA + 500 mg/1 activated charcoal was found best with 96% per cent rooting and 5.42 mean number of roots. Further the regenerated plants were transferred to sterilized cocopit in poly house. After 1 week, plants were transferred to sterilized earthen pots containing a mixture of sand, FYM, soil (1:2:1) for acclimatization and transfer to the open field and 85 per cent plants were survived.


Keywords: Micropropogation, shoot proliferation, acclimatization, and Aloe vera.
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How to cite this article:

Shashi Kiran, Alice Tirkey, Zenu Jha and Porte, S.S. 2017. In–Vitro Regeneration of Aloe Vera (Aloe barbadensis Mill).Int.J.Curr.Microbiol.App.Sci. 6(11): 1829-1834. doi: https://doi.org/10.20546/ijcmas.2017.611.218