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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1409-1418
DOI: https://doi.org/10.20546/ijcmas.2017.611.168


Production of Cellulases by Solid State Fermentation of Different Agricultural Residues Using Humicola insolens MTCC 1433
Diksha Singla1* and Monica Sachdeva Taggar2
1Department of Biochemistry, Punjab Agricultural University, Ludhiana-141004, Punjab, India
2School of Renewable Energy Engineering, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

The production of cellulases is an important strategy for the development of sustainable second-generation ethanol production processes. The present study was undertaken to select cheap and efficient agricultural residue suitable for cellulase production using fungus Humicola insolens. Six different agricultural residues viz., paddy straw, soybean pod husk, sugarcane bagasse, groundnut shells, corn stalks and pigeonpea pod husk were used as substrate in the solid state fermentation medium. Among all the substrates, maximum filter paper (FPase), carboxymethyl cellulase (CMCase), cellobiase and xylanase activities of 71.28 nmol/min/gds, 227.33 nmol/min/gds, 101.02 nmol/min/gds, 1988.62 nmol/min/gds respectively were recorded at 72 hours after incubation using soybean pod husk as substrate, followed by sugarcane bagasse with 58.78 nmol/min/gds FPase, 225.55 nmol/min/gds CMCase, 96.10 nmol/min/gds cellobiase and 1727.94 nmol/min/gds xylanase activities. Soybean pod husk showed maximum cellulase activity as compared to all comparatively other residues.


Keywords: Agricultural residues, Solid state fermentation, FPase, CMCase, Cellobiase, Xylanase.
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How to cite this article:

Diksha Singla and Monica Sachdeva Taggar. 2017. Production of Cellulases by Solid State Fermentation of Different Agricultural Residues Using Humicola insolens MTCC 1433.Int.J.Curr.Microbiol.App.Sci. 6(11): 1409-1418. doi: https://doi.org/10.20546/ijcmas.2017.611.168