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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 1311-1318
DOI: https://doi.org/10.20546/ijcmas.2017.611.157


Callus Induction and Indirect Regeneration of Strawberry (Fragaria × Ananassa) Duch. CV. Chandler
Suvalaxmi Palei1*, G.R. Rout2, A.K. Das1 and D.K. Dash1
1Department of Fruit Science and Horticulture Technology
2Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar, Odisha, India
*Corresponding author
Abstract:

An experiment on callus induction and indirect regeneration of strawberry cv. Chandler was conducted by taking different explants (internodal cuttings, matured leaves, young leaves, flower buds) were cultured on MS medium supplemented with different growth regulators and incubated in culture room for callus induction. Expansion and swelling of explants at the cut surface were noted and soft crystal, greenish compact calli were emerged from the cut surface within 3-4 weeks culture on MS medium supplemented with 2, 4- D (1.0 mg/l) along with BAP (0.1 mg/l). Also same medium resulted the highest callus induction (170.6 mg) along with 90 % callus induction frequency. Healthy calli were taken in a sterile petridish and chopped into small pieces with a fresh weight of approximately 100 - 120 mg, then inoculated on shoot regeneration medium containing different concentration of BAP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg/l) and NAA (0.2 and 0.5 mg/l). Green shoot primordia developed on the surface of calli within 4-5 weeks. The number of shoots formed per callus was investigated after 30 days of culturing. BAP @2.5mg/l and NAA @0.5mg/l with the basal MS media showed the maximum response to regeneration via calli inducing an average of 5.1 shoot buds per culture within 4-5 weeks. Regenerated shoots were then transferred to MS medium with BAP and NAA for shoot multiplication and subsequent induction of best roots. The maximum percentage of rooting was observed in half strength MS media supplemented with 0.25 mg/l IBA and 2% sucrose. The developed plantlets through in vitro culture technique were acclimated and successfully transferred to in vivo condition for further evaluations. Analysis was carried out in completely randomized design with three replications for each treatment.


Keywords: Strawberry, Callus, Plant growth regulators.
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How to cite this article:

Suvalaxmi Palei, G.R. Rout, A.K. Das and Dash, D.K. 2017. Callus Induction and Indirect Regeneration of Strawberry (Fragaria × Ananassa) Duch. CV. Chandler.Int.J.Curr.Microbiol.App.Sci. 6(11): 1311-1318. doi: https://doi.org/10.20546/ijcmas.2017.611.157