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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 956-963
DOI: https://doi.org/10.20546/ijcmas.2017.611.112


Antibiotics Efficacy on In Vitro Growth Parameters of Important Potato Cultivars
Tanuja Buckseth1*, R. K. Singh1, Sumita Sharma1, Ashwani K. Sharma2, Vaishali Moudgil1 and Aastha Saraswati1
1ICAR-Central Potato Research Institute, Shimla- 171001 (HP), India
2ICAR-Central Potato Research Station, Kufri-171012 (HP), India
*Corresponding author
Abstract:

The use of plant tissue culture as a routine method of potato seed production would be costly but these techniques can be used to eliminate the pathogens, produce required initial material and then, use another efficient and cheaper system to rapidly produce high quality seed tubers for commercial production. Considering this, the liquid formulation of antimycotic 100x, and amphotericin-B 250 µg/ml of PAA make was used. These test antibiotics were added aseptically at various concentrations (0.5%, 1%, 1.5%, and 2%) in the sterilized MS medium culture tubes to find out contamination free, vigorous in-vitro growth of the microplants. It was observed that amphotericin-b at lower conc. i.e., 0.5% and 1% could be incorporated in the micro propagation medium of potato to minimize fungal contaminations as well as for improving the microplants vigour. However, all the tested antimycotic concentrations had negative effects on almost all the morphological characters studied, which need further investigation with lower concentrations.


Keywords: Microplants, Antibiotics, Amphotericin-b, Antimycotic, Varieties, Potato.
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How to cite this article:

Tanuja Buckseth, R.K. Singh, Sumita Sharma, Ashwani K. Sharma, Vaishali Moudgil and Aastha Saraswati. 2017. Antibiotics Efficacy on In Vitro Growth Parameters of Important Potato Cultivars.Int.J.Curr.Microbiol.App.Sci. 6(11): 956-963. doi: https://doi.org/10.20546/ijcmas.2017.611.112