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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 925-931
DOI: https://doi.org/10.20546/ijcmas.2017.611.108


Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute Retting
Minakshi Nath, B. Majumdar*, Suparna Das, Sonali P. Mazumdar, A.R. Saha and S. Sarkar
Division of Crop Production, ICAR-Central Research Institute for Jute & Allied Fibres, Barrackpore, Kolkata, West Bengal, 700 120, India
*Corresponding author
Abstract:

Three pectinolytic microbial consortia developed for faster retting of jute (Chorchorus olitorius L. and Chorchorus capsularis L.) were subjected to various pH (4 to 12) and media with variable nitrogen and fixed carbon sources for optimization of fermentation conditions of the consortia for cost-effective pectin degrading enzyme production. All the microbial consortia were active over a wide range of pH from 6 to 10 and recorded maximum polygalacturonase and pectin lyase activities at pH 10. The polygalacturonase and pectin lyase activities of microbial consortium 3 were higher by 2.05 and 3.59 times over microbial consortium 1 and 3.66 and 4.72 times over microbial consortium 2 at pH 10. Among the eight media under study, the yeast extract pectin agar media recorded higher polygalacturonase and pectin lyase production by all the three pectinolytic microbial consortia. Yeast extract was found as the most suitable source of nitrogen over other source of nitrogen and suppliments for polygalacturonase and pectin lyase production. The polygalacturonase and pectin lyase production in yeast extract pectin agar media by microbial consortium 3 were higher by 2.2 and 3.3 times over microbial consortium 1 and 2.8 and 3.94 times over microbial consortium 2. Yeast extract pectin media at alkaline pH (8-10) recorded the maximum polygalacturonase and pectin lyase enzyme production by pectinolytic microbial consortia used for jute retting


Keywords: Fermentation conditions, Pectinolytic microbial consortia, Pectinase production, Jute retting.
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How to cite this article:

Minakshi Nath, B. Majumdar, Suparna Das, Sonali P. Mazumdar, A.R. Saha and Sarkar, S. 2017. Optimization of Fermentation Conditions for Pectin Degrading Enzyme Production by Pectinolytic Microbial Consortia Used for Jute RettingInt.J.Curr.Microbiol.App.Sci. 6(11): 925-931. doi: https://doi.org/10.20546/ijcmas.2017.611.108