National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Three pectinolytic microbial consortia developed for faster retting of jute (Chorchorus olitorius L. and Chorchorus capsularis L.) were subjected to various pH (4 to 12) and media with variable nitrogen and fixed carbon sources for optimization of fermentation conditions of the consortia for cost-effective pectin degrading enzyme production. All the microbial consortia were active over a wide range of pH from 6 to 10 and recorded maximum polygalacturonase and pectin lyase activities at pH 10. The polygalacturonase and pectin lyase activities of microbial consortium 3 were higher by 2.05 and 3.59 times over microbial consortium 1 and 3.66 and 4.72 times over microbial consortium 2 at pH 10. Among the eight media under study, the yeast extract pectin agar media recorded higher polygalacturonase and pectin lyase production by all the three pectinolytic microbial consortia. Yeast extract was found as the most suitable source of nitrogen over other source of nitrogen and suppliments for polygalacturonase and pectin lyase production. The polygalacturonase and pectin lyase production in yeast extract pectin agar media by microbial consortium 3 were higher by 2.2 and 3.3 times over microbial consortium 1 and 2.8 and 3.94 times over microbial consortium 2. Yeast extract pectin media at alkaline pH (8-10) recorded the maximum polygalacturonase and pectin lyase enzyme production by pectinolytic microbial consortia used for jute retting
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