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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 856-864
DOI: https://doi.org/10.20546/ijcmas.2017.611.100


Molecular Detection of Enterotoxigenic E. coli in Milk and Milk Products
W.F. Amin1*, E.H. Ahmed2, M.S. Embarak2, U.H. Abo-Shama3, A.G. Thabit2 and S.Y. Ismail2
1Department of Food Hygiene, Faculty of Veterinary Medicine, Assiut University, Egypt
2 Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt
3Microbiology and Immunology Department, Faculty of Veterinary Medicine,Sohag University, Egypt
*Corresponding author
Abstract:

This study aimed to determine the incidence of E. coli in raw milk and cheese, in addition to isolate and identify Enterotoxigenic E. coli. The existence of stable toxin (STh) and labile toxin (LT) genes were determined in the isolated strains. This study included 350 samples of raw milk and cheese samples. Different media were used to isolate E. coli and different biochemical tests were used for identification. E. coli was detected in 61.4% of samples. They were tested for the presence of STh and LT genes by PCR. ETEC was detected in 3.7% of E. coli isolates. Only one strain from milk of street samples that found to harbor STh gene. Seven strains were detected in Kareish cheese including two strains harbor LT gene and five strains harbor STh gene.


Keywords: Milk, Cheese, E. coli, ETEC, PCR.
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How to cite this article:

Amin, W.F., E.H. Ahmed, M.S. Embarak, U.H. Abo-Shama, A.G. Thabit and Ismail, S.Y. 2017. Molecular Detection of Enterotoxigenic E. coli in Milk and Milk Products.Int.J.Curr.Microbiol.App.Sci. 6(11): 856-864. doi: https://doi.org/10.20546/ijcmas.2017.611.100