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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 6, Issue:11, November, 2017

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 750-758
DOI: https://doi.org/10.20546/ijcmas.2017.611.088


In Vitro Evaluation of Bacterial Biocontrol Agents and Botanicals against Alternaria Leaf Spot Caused by Alternaria macrospora in Cotton
B. Mohan Venkata Siva Prasad1, S.L. Bhattiprolu2*, V. Prasanna Kumari1 and P. Anil Kumar1
1Department of Plant Pathology, Bapatla – 522 101, Andhra Pradesh, India
2Regional Agricultural Research Station, Lam, Guntur – 522 034, Andhra Pradesh, India
*Corresponding author
Abstract:

Four bacterial biocontrol agents (Pseudomonas fluorescens strain 1, strain 2, strain 3 and Bacillus subtilis strain 1) and six botanicals {Neem (Azadirachata indica), Lantana (Lantana camera), Calotropis (Calotropis procera), Tulasi (Ocimum sanctum), Onion bulb (Allium cepa), Garlic glove (Allium sativum)} were evaluated in vitro against Alternaria macrospora causing leaf spot of cotton. All the treatments significantly inhibited mycelial growth of the test fungus over untreated control. Among different bacterial biocontrol agents, B. subtilis strain caused 51.68% inhibition with 1.73cm radial growth of A. macrospora followed by P.f strain 1, P.f strain 3 and P.f strain 2 with radial growth and inhibition of A. macrospora 2.08 cm, 41.89%; 2.15 cm, 39.94% and 2.45 cm, 31.56% respectively. Among botanicals, neem leaf extract was superior to other treatments with 2.83 cm radial growth and 68.52% inhibition of A. macrospora followed by garlic clove with mean radial growth 2.84 cm and 68.40% inhibition of A. macrospora compared to control (9.0 cm).


Keywords: Alternaria macrospora, Cotton leaf spot, Bacterial biocontrol agents, Botanicals.

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How to cite this article:

Mohan Venkata Siva Prasad B., S.L. Bhattiprolu, V. Prasanna Kumari and Anil Kumar, P. 2017. In Vitro Evaluation of Bacterial Biocontrol Agents and Botanicals against Alternaria Leaf Spot Caused by Alternaria macrospora in Cotton.Int.J.Curr.Microbiol.App.Sci. 6(11): 750-758. doi: https://doi.org/10.20546/ijcmas.2017.611.088
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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