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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 693-707
DOI: https://doi.org/10.20546/ijcmas.2017.611.082


Occurrence and Virulence Characterization of Aeromonas hydrophila in Salad Vegetables from Punjab, India
Kamalpreet Kaur1*, Param Pal Sahota1, Mandeep Singh Hunjan2, Bhavish Sood1, Manmeet Kaur1 and Jaspreet Kaur1
1Department of Microbiology
2Department of Plant Pathology, Punjab Agricultural University, Ludhiana-141004, Punjba, India
*Corresponding author
Abstract:

The consumption of fresh and minimally processed vegetables is considered healthy, outbreaks related to the contamination of these products are frequently reported. Present study aimed to evaluate the microbiological quality and the occurrence of A. hydrophila in external, internal and macerated part of salad vegetables (cucumber, radish, carrot, tomato, cabbage, long melon and spinach) from the fields of Punjab Agricultural University and local markets of Ludhiana. Epidemiological surveillance conducted showed the occurrence of A. hydrophila in 82.5% of total tested samples, cucumber (80%), radish (83%), cabbage (100%), carrot (74%), long melon (85%), spinach (100%) and tomato (68.5%). Total plate count ranged from 4.82 to 6.25 log cfu/g. Aerobic plate count of A. hydrophila procured from field and local market ranged between 2.79-3.93 log cfu/g, A. hydrophila count from internal, external and macerated part was 2.54, 3.06 and 3.73 log cfu/g. Isolate were molecularly confirmed as A. hydrophila by 16s -rDNA specific primer. Virulence was confirmed by gene specific primers, act (232 bp) and ahh (130 bp). Results of the study showed that salad vegetables possess a potential risk for the consumers.


Keywords: Aeromonas hydrophila, salad Vegetable, Epidemiological surveillance, Virulence.
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How to cite this article:

Kamalpreet Kaur, Param Pal Sahota, Mandeep Singh Hunjan, Bhavish Sood, Manmeet Kaur and Jaspreet Kaur. 2017. Occurrence and Virulence Characterization of Aeromonas hydrophila in Salad Vegetables from Punjab.Int.J.Curr.Microbiol.App.Sci. 6(11): 693-707. doi: https://doi.org/10.20546/ijcmas.2017.611.082