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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 864-869
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.089


Detection of Carbapenem Resistant Enterobacteriacae from Clinical Isolates
Akshaya Rao* and V.A. Indumathi
Department of Microbiology M.S. Ramaiah Medical College Bangalore, India
*Corresponding author
Abstract:

Carbapenemases are β-lactamases that confer resistance to almost all β-Lactam antibiotics. There is an urgent need for identification of these enzymes. The main aim of this study includes to detect different Carbapenemase enzymes produced by Enterobacteriacae among clinical isolates using phenotypic tests. Isolates from various clinical samples that showed resistance to Imipenem and/or Meropenem by Kirby Bauer disc diffusion method were analysed over a period of 6 months. These isolates were screened using Modified Hodge test (MHT), KPC CHROM agar, and supplemental tests such as Blue Carba Test (BCT) and Inhibitor Based Phenotypic Test (IBPT) were done to detect different Carbapenemase enzymes.  60 out of 430 (13.95%) strains were Carbapenem resistant Enterobacteriacae. Among the 4 tests performed MHT detected Carbapenemases in 36.6% and KPC CHROM agar in 96.6% isolates. Blue Carba Test showed 13.3% isolates as KPC/MBL phenotype and 3.3% as OXA phenotype. Inhibitor Based Phenotypic Test detected 8.3% isolates as KPC producers and 5% as MBL producers. Among these four phenotypic tests KPC CHROM agar could be useful as a screening test for Carbapenemase detection, BCT and IBPT would be useful to differentiate these Carbapenemases.


Keywords: Carbapenemases, KPC Chrom Agar, Modified Hodge Test,Blue Carba Test,Inhibitor Based Phenotypic Test.
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How to cite this article:

Carbapenemases, KPC Chrom Agar, Modified Hodge Test,Blue Carba Test,Inhibitor Based Phenotypic Test.Int.J.Curr.Microbiol.App.Sci. 5(5): 864-869. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.089