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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 506-513
DOI: https://doi.org/10.20546/ijcmas.2017.611.061


Evaluation and Comparison of Currently Available Fluorescence Immunoassay (FIA) Test with Rapid Immuno Chromatographic Test (ICT) Considering NS 1 ELISA as a Reference Test
J.A. Pohekar*, M.H. Bhalchandra, H.S. Ghogre and T.G. Pathrikar
Department of Microbiology, MGM’s Medical College and Hospital, Aurangabad, India
*Corresponding author
Abstract:

Dengue is one of the rapidly emerging diseases globally. Early diagnosis can improve clinical outcome by ensuring close follow up, initiating appropriate supportive treatment and increasing awareness of hemorrhage and shock. Many diagnostic kits are available commercially. Amongst these gold standard test is IgM and IgG ELISA with seroconversion, but we can advice this test only after 5 days when the antibodies will start developing. Nonstructural glycoprotein 1 (NS-1) is considered to be a useful marker for early diagnosis of dengue, as this can be advised early. NS 1 ELISA is carried out only in institutional laboratories where technical expertisation as well as specific machines are available. So, for a routine laboratory with limited resources rapid ICT test is a better choice. In the present study the currently available Fluorescence immunoassay (FIA) test is evaluated and compared with rapid ICT considering NS 1 ELISA as a reference test. To evaluate the currently available fluorescence immunoassay test for detection of Dengue NS 1 antigen. To compare the currently available Fluorescence immunoassay (FIA) test for detection of Dengue NS 1 antigen with rapid ICT test and Microwell ELISA test. Cross sectional observational study. In this study, 200 samples from dengue suspected patients are tested for presence of NS 1 antigen by rapid ICT test. From these 50 positive and 50 negative samples are further retested by NS 1 ELISA and NS 1 by FIA. Results are analyzed and compared with each other. NS 1 ELISA is considered as a reference test and NS 1 Fluorescence immunoassay and rapid ICT as an index tests. Data is analyzed and sensitivity, specificity, positive predictive value and negative predictive value are derived for each test. FIA test is more specific and having positive predictive value of 97.43% which states that it is more reliable than rapid ICT. FIA is less time consuming and easy to perform whereas Microwell ELISA is time consuming and require highly technical expertise. NS 1 Microwell ELISA and FIA results are very much comparable with each other. Whereas false positive less with FIA and more with rapid ICT card test.


Keywords: Dengue, FIA, ELISA, ICT, NS 1 Antigen.
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How to cite this article:

Pohekar, J.A., M.H. Bhalchandra, H.S. Ghogre and Pathrikar, T.G. 2017. Evaluation and Comparison of Currently Available Fluorescence Immunoassay (FIA) Test with Rapid Immuno Chromatographic Test (ICT) Considering NS 1 ELISA as a Reference Test.Int.J.Curr.Microbiol.App.Sci. 6(11): 506-513. doi: https://doi.org/10.20546/ijcmas.2017.611.061