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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 494-499
DOI: https://doi.org/10.20546/ijcmas.2017.611.059


Diagnosis of Subclinical Endometritis in Murrah Buffaloes through Cytobrush Technique
Ravi Dutt1*, Gyan Singh2, Mahavir Singh3, Maneesh Sharma2, Jasmer Dalal1 and R.K. Chandolia1
1Department of Veterinary Gynaecology and Obstetrics
2Department of Veterinary Clinical Complex
3College Central Laboratory, Lala Lajpat Rai University of Veterinary and Animal Sciences Hisar-125004, Haryana, India
*Corresponding author
Abstract:

The objective of this study was to find out microbial cause of repeat breeding in ten repeat breeder Murrah buffaloes at Government Livestock Farm, Sector-II, Hisar. The cytological samples were obtained from these buffaloes’ uterus using cytobrush technique and relating the findings to the presence of polymorphonuclear cells, identification of bacteria and antibiotic sensitivity pattern is reported. The subclinical endometritis was declared on the basis of presence of ≥ 10% polymorphonuclear cells in endometrial smears. Besides Bacillus (5), mixed infection of Staphylococci + E. coli (3), Bacillus + E. coli (1), and Proteus + Bacillus (1) was also recorded. There was highest prevalence of Bacillus (46.66%) followed by E. coli (26.67 %), Staphylococci (20.0%) and Proteus (6.66%). The bacterial isolates reported in the present study showed highest sensitivity (60%) towards Moxifloxacin and Enrofloxacin followed by Chloramphenicol and Gentamicin (33.3%) and least towards Ceftriaxone and Cefoperazone (13.3%). All the isolates showed resistance towards Penicillin-G, Ofloxacin, Amoxicillin, Oxytetracycline, Ampicillin, Neomycin, Amikacin, Cloxacillin and Cephalexin.The findings of the present study revealed that cytobrush technique could preferentially be used to detect subclinical endometritis in clinical practice.


Keywords: Buffalo, Cytobrush, Cytology, Endometritis, Murrah, Subclinical.
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How to cite this article:

Ravi Dutt, Gyan Singh, Mahavir Singh, Maneesh Sharma, Jasmer Dalal and Chandolia, R.K. 2017. Diagnosis of Subclinical Endometritis in Murrah Buffaloes through Cytobrush Technique.Int.J.Curr.Microbiol.App.Sci. 6(11): 494-499. doi: https://doi.org/10.20546/ijcmas.2017.611.059