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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 840-847
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.086


Protective effect of Ocimum sanctum plant extract against DNA damage induced by malathion in cultured human peripheral blood lymphocytes
Neeraj Kumar1, Anita Yadav1*, Neeraj Aggarwal2 and Ranjan Gupta3
1Department of Biotechnology, Kurukshetra University Kurukshetra-1136119 (Haryana), India
2Department of Microbiology, Kurukshetra University Kurukshetra-1136119 (Haryana), India
3Department of Biochemistry, Kurukshetra University Kurukshetra-1136119 (Haryana), India
*Corresponding author
Abstract:

Pesticides are ubiquitous contaminants of our environment and are extensively used all over the world. Malathion is an organophosphate pesticide used to fight against insects in the crop fields. Besides providing protection against insects it also has harmful effects on humans. Its toxicity has been associated with the inhibition of acetyl cholinesterase activity, leading to the interference with the transmission of nerve impulse, accumulation of acetylcholine at synaptic junctions, and subsequent induction of adverse health effects. Keeping in mind the adverse effects of malathion, we therefore studied the protective effect of Ocimum sanctum extract against the malathion induced DNA damage using comet assay as a biomarker of genotoxicity. To observe the protective effect of Ocimum sanctum extract, heparinised fresh blood from healthy individuals was treated with 30 µg/ml of malathion in presence of ocimum extract. Ocimum extract at the concentration of 40 µg/ml and 70 µg/ml had significantly reduced (p<0.05) the tail moment (TM) as compared to malathion exposed sample. Hence, our findings suggest that Ocimum sanctum extract has protective effect against DNA damage induced by malathion in cultured human peripheral blood lymphocytes.


Keywords: Malathion,Comet assay, Tail moment, Ocimum sanctum extract, Human peripheral blood lymphocytes
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How to cite this article:

Neeraj Kumar, Anita Yadav, Neeraj Aggarwal and Ranjan Gupta. 2016. Protective effect of Ocimum sanctum plant extract against DNA damage induced by malathion in cultured human peripheral blood lymphocytesInt.J.Curr.Microbiol.App.Sci. 5(5): 840-847. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.086