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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(11): 132-134
DOI: https://doi.org/10.20546/ijcmas.2017.611.017


Standardization of Isolation Technique of Rhizobium from Root Nodules of Lentil
Ajay Varun1, Arun Patel2, Umakant Banjare3*, Santosh Kumar3 and Ramesh Kumar Singh3
1Biotechnology, Rajeev Gandhi South Campus, Barkachha, Mirzapur, India
2Department of Botany, Institute of Science, B.H.U. Varanasi, India
3Department of Genetics and Plant Breeding, Institute of Agricultural Sciences,B.H.U. Varanasi, India
*Corresponding author
Abstract:

Native plant growth promoting rhizobacteria are unable to compete with other bacterial strains and hence cannot express their full potential in realising the beneficial effects. Hence, inoculation of plants with target microorganism is necessary to exploit full potential for yield enhancement. This study was aimed at standardizing the technique for isolation of Rhizobium species from root nodules of lentil so as to explore and maximize their contributions to soil nitrogen fertilization in place of synthetic fertilizers. A total of five nodulated healthy plants collected from North-eastern part of Uttar Pradesh, India were sterilized and macerated under aseptic condition followed by streaking one loop full suspension in a zig-zag manner using the inoculation loop on YEMA medium in laminar air flow and incubated in a B.O.D. incubator at 28±2ºC for 24 hours. Growth on YEMA plate was observed after the said incubation period and then maintained at 4ºC in 20% (w/v) glycerol at -80ºC for long-term storage. Vigorous growth of bacteria was seen in next 24 to 48 hours. Bacterial colonies were found to have distinct colour, morphology, appearance and texture on agar plates.


Keywords: Rhizobacteria, YEMA, PGPR, Agar, Lentil.
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How to cite this article:

Ajay Varun, Arun Patel, Umakant Banjare, Santosh Kumar and Ramesh Kumar Singh. 2017. Standardization of Isolation Technique of Rhizobium from Root Nodules of Lentil.Int.J.Curr.Microbiol.App.Sci. 6(11): 132-134. doi: https://doi.org/10.20546/ijcmas.2017.611.017