Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 488-497
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.049


Evaluation of Bioactive chemical constituents by Gas chromatography-Mass spectrometry analysis isolated from Bacillus species
K. Maheshwari, K. Saraswathi, D. Sankari and P. Arumugam
Department of Biotechnology, SRM University, SRM Nagar, Kattankulathur-603 203, Kancheepuram District, Tamilnadu, India
Armats Biotek Training and Research Institute, Guindy, Tamilnadu, India
*Corresponding author
Abstract:

The aim of the research study concerns on screening, production and partial purification of Secondary metabolites isolated from soil sample collected from Western ghats, India. The sample was been serially diluted and plated on Nutrient Agar and bacteria were selectively isolated. The broth culture of the nutrient medium was preferred for fermentation process and extracellular metabolites were been extracted using the solvent ethyl acetate. The crude compound (ABTRI 3 strain) was been primarily screened for antimicrobial activity and based on the activity potent strain was been selected for secondary metabolites production. Secondary metabolites showed distinct bands by thin layer chromatography and maximum inhibition was observed at final concentration for antibacterial activity. The potential strain was characterized by GCMS and identified as Bacillus sp. by 16s rRNA sequencing. The findings of the present study suggested that the selected isolate could be a potent natural source of bioactive compounds


Keywords: Fermentation process, Antimicrobial activity, 16srRNA sequencing, Bacillus sp
Download this article as Download

How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 488-497. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.049