National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The aim of the research study concerns on screening, production and partial purification of Secondary metabolites isolated from soil sample collected from Western ghats, India. The sample was been serially diluted and plated on Nutrient Agar and bacteria were selectively isolated. The broth culture of the nutrient medium was preferred for fermentation process and extracellular metabolites were been extracted using the solvent ethyl acetate. The crude compound (ABTRI 3 strain) was been primarily screened for antimicrobial activity and based on the activity potent strain was been selected for secondary metabolites production. Secondary metabolites showed distinct bands by thin layer chromatography and maximum inhibition was observed at final concentration for antibacterial activity. The potential strain was characterized by GCMS and identified as Bacillus sp. by 16s rRNA sequencing. The findings of the present study suggested that the selected isolate could be a potent natural source of bioactive compounds
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