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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 767-787
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.078


Screening and Characterization of Alkaline Protease Producing Bacillus Strain B-4 Bacillus flexus and Study of its Potential for Alkaline Protease Production
Shilpa A. Jani1*, Yesha M. Parekh2, Tanvi N. Parmar2, Tulsi J. Dalwadi2, Hetal B. Patel1 and Sagar K. Parmar2
1Microbiology Department, J and J college of Science, Nadiad, Gujarat, India
2School of Sciences, Gujarat University, Ahmedabad, Gujarat, India
*Corresponding author
Abstract:

Microbial proteases account for approximately 40% of the total worldwide enzyme sales and most of the commercial proteases are of bacterial origin. So planned to screen out bacterial cultures from various sources producing significant amount of alkaline proteases. Samples collected were serially diluted and plated on skim milk agar plates and incubated at 37 ºC for 3-4 days to allow the colonies to grow. Zone of clearance of casein surrounding the colony has provided a measure of their Proteolytic activity. The isolates showing zone of casein hydrolysis on milk agar plates were studied for their relative enzyme activity (REA) on skim milk agar plate. To compare the caseinolytic activity of enzyme produced, the isolates were inoculated in production medium and put on an environmental shaker at 100 rpm at 37ºC for 168 hours and checked for enzyme activity at interval of 24 hours. Selected isolateB-4 was studied for cultural, morphological, biochemical   characteristics and identified by 16S rRNA sequencing and also employed for optimization of cultural conditions and components of production medium. We also employed the crude enzyme for its efficiency of gelatin hydrolysis and silver extraction from used X-ray films.


Keywords: Alkaline protease, Bacillus,Relative enzyme activity, optimization.
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How to cite this article:

Shilpa A. Jani, Yesha M. Parekh, Tanvi N. Parmar, Tulsi J. Dalwadi, Hetal B. Patel and Sagar K. Parmar. 2016. Screening and Characterization of Alkaline Protease Producing Bacillus Strain B-4 Bacillus flexus and Study of its Potential for Alkaline Protease Production.Int.J.Curr.Microbiol.App.Sci. 5(5): 767-787. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.078