Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 478-487
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.048


Exploration of Extracellular Phytase Production by Amycolatopsis vancoresmycina S-12 in Submerged Fermentation
Debashis Bandyopadhyay, Kamala Das and Sukanta K Sen
Microbiology Division, Department of Botany, Visva-Bharati, Santiniketan-731235, India
*Corresponding author
Abstract:

In an attempt to explore extracellular phytase producing Actinomycete, the stain S-12 a soil isolate was selected on the basis of clearing zone formation on PSM plate and by assaying of fermented broth. The isolate S-12 identified as Amycolatopsis vancoresmycina through morphological, cultural and molecular characterization. As the fermentation process is significantly influenced by various physical and chemical parameters, hence, the isolate was tested in varied fermentation conditions. The maximum production of phytase was observed at 120 hour of incubation at 37°C and at pH 6.5. The most suitable carbon and nitrogen sources were 1.5% glucose and 1% potassium nitrate, supplementary carbon source 0.5% Na-Phytate. As surfactant, Tween 80 (0.5%) showed enhancement of phytase production. Notably, the A. vancoresmycina S-12 is able to enhance phytase production close to 8 times by medium optimization only at its wild state.


Keywords: Phytase; Antinutrients; Fermentation; Optimization; Amycolatopsis vancoresmycina
Download this article as Download

How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 478-487. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.048