National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
In an attempt to explore extracellular phytase producing Actinomycete, the stain S-12 a soil isolate was selected on the basis of clearing zone formation on PSM plate and by assaying of fermented broth. The isolate S-12 identified as Amycolatopsis vancoresmycina through morphological, cultural and molecular characterization. As the fermentation process is significantly influenced by various physical and chemical parameters, hence, the isolate was tested in varied fermentation conditions. The maximum production of phytase was observed at 120 hour of incubation at 37°C and at pH 6.5. The most suitable carbon and nitrogen sources were 1.5% glucose and 1% potassium nitrate, supplementary carbon source 0.5% Na-Phytate. As surfactant, Tween 80 (0.5%) showed enhancement of phytase production. Notably, the A. vancoresmycina S-12 is able to enhance phytase production close to 8 times by medium optimization only at its wild state.
Citations |
 |
 |
 |
 |
