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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:5, May, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 636-646
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.064


In Vitro Antidermatophytic Study on Leaf Extracts of Azadirachta indica and Andrographis paniculata against Trichophyton Species
C.Banu Rekha1,2*, G. Krishnaveni2, P. Rajendran3, Ramya Anandan2 and M.Elakkiya2
1Research Scholar, Bharathiyar University, Coimbatore, India
2Department of Microbiology, Dr. MGR Janaki College of Arts & Science for Women, Chennai
3Department of Microbiology, Sri Ramachandra medical College & Research Institute, Sri Ramachandra University, Porur, Tamilnadu, India
*Corresponding author
Abstract:

The study was to assess the antidermatophytic activity of the ethanol, ethyl acetate and aqueous extract of Neem (Azadirachta indica) and Nilavembu (Andrographis paniculata) leaves with ethanol, chloroform and aqueous extract against dermatophytes isolated from different soil. Dermatophytes were identified, Out of the 115 soil samples, 64 isolates of Trichophyton mentagrophytes, 42 isolates of Trichophyton rubrum, 24 isolates of Trichophyton tonsurans, 37 isolates of Trichophyton terrestre, 20 isolates of Trichophyton verrucosum were isolated and identified. We selected two most common pathogen among the Trichophyton species i.e., Trichophyton mentagrophytes and Trichophyton rubrum to find the antidermatophtitic activity of Plant extracts. The minimum inhibitory concentration (MIC) by broth dilution method and minimum fungicidal concentration (MFC) with the leaf extracts of the plants Azadirachta indica and Andrographis paniculata against the isolated dermatophytes was determined. Among the Trichophyton species, T.mentagrophytes was inhibited by ethanolic extract of Azadirachta indica leaves in the concentration of 62.5 (µg/ml), T.rubrum was inhibited by ethanolic extract in the concentration of 125(µg/ml), Ethanolic extract was found to have higher inhibitory activity than the ethyl acetate and the aqueous extract against both the organism T.mentagrophytes and T.rubrum. T.mentagrophytes was inhibited by ethanolic extract of Andrographis paniculata leaves extract in the concentration of 125(µg/ml), T.rubrum was inhibited by ethanolic extract in the concentration of 62.5(µg/ml). Ethanolic extract found to have higher activity than the chloroform and the aqueous extract against both the organism T.mentagrophytes and T.rubrum. T.mentagrophytes and T.rubrum were inhibited by ethanolic extract of Azadirachta indica and Andrographis paniculata extract in the concentration of 62.5 (µg/ml), against T.mentagrophytes and 125(µg/ml), against T.rubrum. Ethanolic extract with the combination of Azadirachta indica and Andrographis paniculata have greater inhibitory activity against T.mentagrophytes than T.rubrum when combined together due to the synergistic or additive effect of the phytocomponents. The results of this research gives us the therapeutic potential of Azadirachta indica and Andrographis paniculata  to control the dermatophytosis.


Keywords: Dermatophytes, Soil sample, Hair baiting technique, Antidermatophytic activity, Azadirachta indica, Andrographis paniculata.

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How to cite this article:

Banu Rekha, C., G. Krishnaveni, P. Rajendran, Ramya Anandan and Elakkiya, M. 2016. In Vitro Antidermatophytic Study on Leaf Extracts of Azadirachta indica and Andrographis paniculata against Trichophyton Species.Int.J.Curr.Microbiol.App.Sci. 5(5): 636-646. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.064
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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