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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 581-592
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.059


Exploration of Plant-Biomass Degrading Fungi for In Vitro
Mycoremediation of Toxic Synthetic Dyes
Raj Kumar Pandey1*, Babita Rana1, Salil Tewari2, Anwesa Sarkar3,
Ashutosh Dubey4, Dinesh Chandra5 and Lakshmi Tewari1
1Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology Pantnagar, U. S. Nagar, Uttarakhand, India
2Department of Genetics and Plant Breeding, College of Agriculture, G.B. Pant University of Agriculture and Technology Pantnagar, U. S. Nagar, Uttarakhand, India
3Department of Post Harvest Process and Food Engineering, College of Technology, G.B. Pant University of Agriculture and Technology Pantnagar, U. S. Nagar, Uttarakhand, India
4Department of Biochemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology Pantnagar, U. S. Nagar, Uttarakhand, India
5Department of Biological Sciences, G. B. Pant University of Agriculture and Technology Pantnagar, U. S. Nagar, Uttarakhand, India
*Corresponding author
Abstract:

The use of extracellular enzyme systems from wood decaying fungi are now growing very fast for bioremediation of synthetic and toxic dyes from environment. In view of this, present study was undertaken for decolorization of two synthetic industrial viz. congo red and brilliant green using wood rot fungal cultures. Fifty five wood rotting fungal cultures were tested qualitatively for production of extracellular lignolytic enzymes. Selected cultures were used for testing the in vitro dye removal potential in dye containing broth. Samples were withdrawn periodically and percent decolorization was calculated. The fungal cultures removed the dye from the media either by accumulating it in mycelia (bio-sorption) or by metabolizing it to some non-coloured components. The cultures varied in their dye decolorizing potential, showing 50.21-97.37% decolorization of brilliant green within 24 d. All the selected cultures showed complete bio-sorption of congo red dye within one month. The efficient strains were further selected for the production of various enzymes involved in the dye decolorization and crude enzyme activities in culture supernatantes were calculated. In all the cases, maximum extracellular laccase, lignin peroxidases and Mn dependent peroxidase activities were observed within 15 to 18 d of incubation in culture supernatant. Light microscopy and phase contrast microscopy clearly revealed bio-sorption of the dye by fungal cultures in the photomicrographs. Among various cultures tested, the potential isolate showing maximum dye decolorizing/ bioabsorbing ability identified as Ganoderma sp.


Keywords: Plant-Biomass,Mycoremediation,Toxic Synthetic Dyes,Ganoderma sps,
congo red and brilliant green.
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How to cite this article:

Raj Kumar Pandey, Babita Rana, Salil Tewari, Anwesa Sarkar, Ashutosh Dubey, Dinesh Chandra and Lakshmi Tewari. 2016. Exploration of Plant-Biomass Degrading Fungi for In Vitro Mycoremediation of Toxic Synthetic DyesInt.J.Curr.Microbiol.App.Sci. 5(5): 581-592. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.059