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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Int.J.Curr.Microbiol.App.Sci.2016.5(5): 448-457
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.047


β-Thalassemia Mutations among Thalassemia Major Patients in Basrah Province - Iraq
Adnan I. Al-Badran1*, Meaad K. Hassan2 and Assad F. Washil1
1Department of Biology, College of Science, University of Basrah, Iraq
2CABP, Professor, Department of Pediatrics, College of Medicine, University of Basrah, Iraq
*Corresponding author
Abstract:

ß- thalassemia is one of the important health problems in Basrah, Southern Iraq were the frequency of carriers for ß- thalassemia gene reaches 4.6%. The study aimed to define the types of mutation among patients with ß- thalassemia major (ß-TM) registered at the Center for Hereditary Blood Diseases (CHBD) and geographical distribution of these mutations in different districts of Basrah. Blood samples were collected from 100 known patients with β-TM (53 males and 47 females), their ages ranged from 1-31 year. The amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique was used for molecular diagnosis of seven types of β-thalassemia mutations codon15 (G-A), IVS1nt -5(G-C), codon 8/9(+G), codon 30(G-C), -88(C-T), codon41/42(-TCTT) and codon 8(-AA). The most frequent β- thalassemia mutations are codon-15(G-A) and IVS 1nt-5(G-C) in 31 (37.3%) and 18(21.7%) of patients respectively, followed by codon 8/9 in 14(16.9%) and codon 30 in 8 (9.6%). In addition -88 (C-T) and Codon 41/42(-TCTT) were reported in 10(12.1%) and 2(2.4%) of patients respectively. Codon 8(-AA) was not reported in this study. The frequencies of these mutant genes differ from that of other parts of Iraq and neighboring countries. The study confirms a heterogeneity of diagnosed mutations within different districts of Basrah which have been studied for the first time in this province and signifies the need for further future studies to detect mutations that were not identified in this study.


Keywords: Mutation, β- thalassemia, ARMS-PCR, Basra,Iraq.
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How to cite this article:

Adnan I. Al-Badran, Meaad K. Hassan and Assad F. Washil. 2016. β-Thalassemia Mutations among Thalassemia Major Patients in Basrah Province – IraqInt.J.Curr.Microbiol.App.Sci. 5(5): 448-457. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.047