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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 430-439
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.045


Prevalence and Antimicrobial Susceptibility of Extended Spectrum β-Lactamases (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolates in a Tertiary Care Hospital in North-West India
Saroj Hooja*, Nita Pal, Rajendra Karadiya, Rajni Sharma, Ramesh Mishra and Rakesh Maheshwari
Department of Microbiology SMS Medical College, Jaipur, India
*Corresponding author
Abstract:

Infections caused by extended spectrum β-lactamases (ESBL) producing gram negative bacteria complicate therapy and limit treatment options. The aim of the present study was to determine the prevalence and susceptibility of ESBL Escherichia coli (E. coli) and Klebsiella pneumoniae from various clinical isolates among hospitalized and outdoor patients at our tertiary care hospital. A total of 412 isolates from various clinical samples were screened for ESBL as per CLSI guidelines and confirmed by Phenotypic Confirmatory Disc Diffusion Test (PCDDT) and Double Disc Synergy Test (DDST). Antimicrobial susceptibility of these isolates was performed by Kirby- Bauer disc diffusion method. ESBL production was seen in 174/281(61.92%) of E. coli and 74/131(56.48%) of Klebsiella pneumoniae isolates. PCDDT detected 174(61.3 %) and 74(56.5%) of ESBL while DDST detected 164 (58.4%) and 70 (53.4%) of ESBL E. coli and Klebsiella pneumoniae respectively. ESBL producers were higher among inpatients 199/323(61.60%) compared to outpatients 49/89(55.05%). ESBL production was found to be highest among blood isolates. A high level of resistance was seen with third generation cephalosporins, piperacillin and cefepime in both ESBL E. coli and Klebsiella pneumoniae isolates. Strict adherence to the hospital antibiotic policy and good infection control practices would go a long way in curtailing the menace of drug resistance.


Keywords: DDST,Escherichia coli, extended spectrum β-lactamases, Klebsiella pneumoniae, PCDDT.
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How to cite this article:

Saroj Hooja, Nita Pal, Rajendra Karadiya, Rajni Sharma, Ramesh Mishra and Rakesh Maheshwari. 2016. Prevalence and Antimicrobial Susceptibility of Extended Spectrum β-Lactamases (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolates in a Tertiary Care Hospital in North-West India.Int.J.Curr.Microbiol.App.Sci. 5(5): 430-439. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.045