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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 275-283
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.030


Biochemical Detection of Phenolic Compounds and Peroxidase in New Locally Improved Tomato Lines by using HPLC
Mosaad F. Ahmed*, Hanafy A. Hamza, Ibrahiem A. Ibrahim, A.A. Nower and Mostafa Alansary
Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Egypt
*Corresponding author
Abstract:

Fifteen different tomato genotypes obtained from breeding program were screened to evaluate their tolerant potential toward Fusarium wilt disease under green house conditions. Relation between tolerant potential of tested tomato genotypes toward Fusarium oxysporum fsp.lcyopersici and accumulation of peroxidase enzyme as well as polyphenolic compounds was achieved through bio-chemical analysis investigations using high pressure liquid chromatogram (HPLC) under in vitro conditions. The results showed that the most tolerant tomato genotypes to pathogenic Fusarium showed the highest ability to produce either peroxidases or polyphenolic compounds under the test conditions. Thus the accumulation of antioxidant enzymes, peroxidases, and polyphenols was negative proportionally with reduction of Fusarium wilt disease severity. Although, the highest growth criteria were recorded within genotypes produced the highest amount of both peroxidase and polyphenols. In conclusion, significant variation among the obtained tomato genotypes from the breeding program was detected with regard to their natural tolerant ability against Fusarium oxysporum.  According to bio-chemical assay using HPLC, there is, for some extent, an evidence for positive correlation between natural ability of tested tomato genotypes to produce peroxidase and polyphenols compounds and the resistance against Fusarium wilt disease which point to a possible correlation between phenolic and peroxidase anti oxidant enzyme and tolerance potential of tomato genotypes toward hazardous pathogens like Fusarium species.


Keywords: Tomato breeding,Fusaruim  resistance phenolic compounds peroxidases activity HPLC.
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How to cite this article:

Mosaad F. Ahmed, Hanafy A. Hamza, Ibrahiem A. Ibrahim, A.A. Nower and Mostafa Alansary. 2016. Biochemical Detection of Phenolic Compounds and Peroxidase in New Locally Improved Tomato Lines by using HPLC.Int.J.Curr.Microbiol.App.Sci. 5(5): 275-283. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.030