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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3757-3767
DOI: https://doi.org/10.20546/ijcmas.2017.609.464


Antioxidant Properties and Antimicrobial Potential of Aqueous Extract of Basidioma from Lentinus edodes (Berk.) Sing. (Shiitake)
Jéssica Daiane de Melo Macoris1, Tatiane Brugnari1, Cinthia Gandolfi Boer2, Alex Graça Contato1*, Rosane Marina Peralta1 and Cristina Giatti Marques de Souza1
1Laboratory of Biochemistry of Microorganisms, Department of Biochemistry, State University of Maringá, 87020-900, Maringá, PR, Brazil
2Laboratory of Clinical Citology, Department of Clinical Analysis and Biomedicine, State University of Maringá, 87020-900, Maringá, PR, Brazil
*Corresponding author
Abstract:

Several scientific studies have proven that mushrooms are foods of high nutritional and medicinal value. It is also proven that mushrooms have bioactive compounds with therapeutic properties, such as the ability to produce antioxidant substances. The objective of this study was to evaluate the antioxidant, antimicrobial and chemical composition of the aqueous extract of dried Shiitake mushrooms obtained from local trade. Four methods were used for analysis of antioxidant activity: ABTS, DPPH, reducing power and chelating activity of Fe+2. The antimicrobial activity was evaluated against bacteria and yeasts. The extraction yield was 25% and the extract presented 5.66 mg/g dry mushroom in total phenolics, 170.96 mg/g in proteins and 380.29 mg/g in total carbohydrates. The lowest EC50 value found was for the ABTS method (0.201 mg/mL), but all methods showed a strong correlation with total phenolics. Some bacteria were sensitive to the extract and the two yeasts tested had a low minimum inhibitory concentration.


Keywords: L. edodes, antioxidant assays, MIC, mushroom, antimicrobial
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How to cite this article:

Jéssica Daiane de Melo Macoris, Tatiane Brugnari, Cinthia Gandolfi Boer, Alex Graça Contato, Rosane Marina Peralta and Cristina Giatti Marques de Souza. 2017. Antioxidant Properties and Antimicrobial Potential of Aqueous Extract of Basidioma from Lentinus edodes (Berk.) Sing. (Shiitake).Int.J.Curr.Microbiol.App.Sci. 6(9): 3757-3767. doi: https://doi.org/10.20546/ijcmas.2017.609.464