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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 218-226
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.024


Biochemical and Molecular Characterization of Efficient Phytase Producing Bacterial Isolates from Soil Samples
Sona S. Dev*, Nisha E.A. and Akhil Venu
Department of Biotechnology, St Peter’s College, Kochi, India
*Corresponding author
Abstract:

Phytase unavailability in monogastric livestocks leads to the release of undigested phytate phosphorus in their faeces and urine causing severe ecological problems. Phytic acid (or phytate) also acts as an anti-nutritional chelating agent for various metal ions, thus reducing the nutritive quality of food. Hence phytase supplementation can be used to reduce the environmental problem as well as improve the bioavailability of various minerals in food.  In the present study  efforts were made to isolate, screen and characterize efficient phytase producing strains of bacteria from different soil samples in Central Kerala, India. A total of 42 bacterial isolates were recovered and tested for their phytase activity and three of them, S13 (1), S13 (4) and S14 showed significant phytase activity on Phytase Screening Medium (PSM) agar plate.  The isolates were initially identified by morphological and biochemical analysis. Amplification of 16SrRNA gene using specific primers, nucleotide homology analysis and phenotypic characterization   revealed that these isolates showed homology to Bacillus sp, Pseudomonas sp. and Lysinibacillus sp. respectively. Optimization of culture conditions for maximum phytase production was also carried out. This is the first study that reports the production of phytase from Lysinibacillus sp. isolated from soil samples.


Keywords: Phytase,Phytic acid,PSM,16SrRNA Lysinibacillus sp.
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How to cite this article:

Sona S. Dev, Nisha E.A. and Akhil Venu. 2016. Biochemical and Molecular Characterization of Efficient Phytase Producing Bacterial Isolates from Soil Samples.Int.J.Curr.Microbiol.App.Sci. 5(5): 218-226. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.024