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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3604-3613
DOI: https://doi.org/10.20546/ijcmas.2017.609.443


Modification of Glycan Expression in Staphylococcus epidermidis to Prevent Biofilm Formation
Ekpiken Solomon Ekpiken*, Lawrence B. Etim and Mercy Ekong
Department of Biological Sciences Cross River University of Technology, Nigeria
*Corresponding author
Abstract:

Staphylococcus epidermidis is a common commensal of the skin and mucous membranes of humans. However, it is a common cause of nosocomial infection especially infection associated with the insertion of biomedical devices. Glycans have been shown to mediate vast majority of biological processes and its alterations have been reported to be used as biomarkers and applied in diagnosis of malignancies and infections. The absolute mean Optical Density (OD450nm) of the biofilm was calculated as 0.248± 0.044SD and the biofilm was observed to strongly adhere to the microtitre plate surface. The biofilm growth curve peaks at 2hrs with a mean OD450nm of 0.385; at 30 minutes and 4hrs growth, mean OD450nm were 0.365 and 0.383 respectively and drastically drops to 0.248 at 24hrs. A panel of 11 biotinylated lectins was used to detect any alterations in glycosylation of the glycans expressed by of S. epidermidis RP62A (ATCC 35984). Glycans’ expression varies with time and most glycans showed strong expression at 2hrs of incubation except N-acetyl galactosamine (GalNAc), which showed strong expression at 30 minutes. Glycosylation inhibitors such as Neuraminidase, Cytidine 5’- monophospho-N- acetylneuraminic acid sodium salt (CMP-NANA), Sialic acid aldolase and 1M Hydrochloric acid were used to prevent biofilm establishment and development and their effects evaluated using toluidine blue stain and lectin screening. The glycosylation inhibitors showed remarkable 80% reduction in Extracellular Polysaccharides (EPS) production and the expression of glycans in comparison with the control, and were most effective at 2hrs relative to 0hr and 4hrs of incubation except 1M HCl, which was effective at 0hr compared to other times of incubation. This study reveals new strategies of using glycosylation inhibitors to prevent biofilm formation during implant of medical devices.


Keywords: Glycosylation inhibitors, Staphylococcus epidermidis, Desialylation; Glycans; enzyme- linked lectin assay (ELLA) and Lectins.
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How to cite this article:

Ekpiken Solomon Ekpiken, Lawrence B. Etim and Mercy Ekong. 2017. Modification of Glycan Expression in Staphylococcus epidermidis to Prevent Biofilm Formation.Int.J.Curr.Microbiol.App.Sci. 6(9): 3604-3613. doi: https://doi.org/10.20546/ijcmas.2017.609.443