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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3567-3584
DOI: https://doi.org/10.20546/ijcmas.2017.609.439


Molecular Characterization of Fusarium Wilt Resistant and Susceptible Safflower (Carthamus tinctorius L.) Genotypes
S.S. Shende* and D.H. Waghmare
Department of Plant Biotechnology, VilasraoDeshmukh College of Agricultural Biotechnology, V.N.M.K.V., Latur (M.S.) India, Pin-413 512
*Corresponding author
Abstract:

Safflower (Carthamus tinctorius L.) is an oilseed crop that is valued as a source of high quality vegetable oil. The present study was carried out with the objective of evaluation of genetic diversity among 11 Fusarium wilt resistance and 5 susceptible safflower genotypes by using RAPD and SSR markers.241 RAPD primer as well as 17 SSR polymorphic primers were used in this study. Analysis diversity showed significant variation in genotypes for the Fusarium wilt disease-related traits. Based on RAPD markers, a total of 1444 bands were amplified and 885 bands (6076%) of these were polymorphic and SSR yielded a total of 20 scorable bands and 19 bands (97.06%) were polymorphic. A logical similarity between the genotype clusters based on molecular data with their resistance or susceptibility to Fusarium wilt was observed.PIC for RAPD markers ranged between 0.50-0.92 with an average of 0.80 and for SSR markers ranged between 0.12-0.96 with an average of 0.63. Therefore, from the present study, it can be proved that RAPD and SSR markers can detect high polymorphism and are very useful in studying variation among different safflower genotypes.


Keywords: Safflower, Fusarium wilt, Genetic diversity, RAPD, SSR.
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How to cite this article:

Shende, S.S. and Waghmare, D.H. 2017. Molecular Characterization of Fusarium Wilt Resistant and Susceptible Safflower (Carthamus tinctorius L.) Genotypes.Int.J.Curr.Microbiol.App.Sci. 6(9): 3567-3584. doi: https://doi.org/10.20546/ijcmas.2017.609.439