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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3235-3246
DOI: https://doi.org/10.20546/ijcmas.2017.609.398


Investigating the Effects of Exogenous Factors on Growth, Photosynthetic Pigments and Bud Induction in Gracilaria corticata var. cylindrica under In vitro Conditions
A. Anuraj1*, Ajit Arun Waman2, Chandra Prakash3, S. Dam Roy4, M. Viji1,Manoj Baidya1 and N.K. Chadha3
1Division of Fisheries Science,
2Division of Horticulture and Forestry, ICAR-Central Island Agricultural Research Institute, Port Blair, India
3Division of Aquaculture, ICAR-Central Institute of Fisheries Education, Mumbai, India
4Director, ICAR-Central Island Agricultural Research Institute, Port Blair, India
*Corresponding author
Abstract:

Effect of kind and concentration of culture media, and plant growth regulators on in vitro response was studied in an economically important seaweed species viz. Gracilaria corticata var. cylindrica. Filter sterilized autoclaved artificial seawater medium at 100 % concentration (A2) was found to be the most optimum for in vitro culture as the cultured explants showed superiority in terms of growth and photosynthetic pigment content, apart from inducing lateral bud formation. Incorporation of cytokinin alone or in combination with auxin (IAA) promoted growth of seaweed explants in A2 media.A2 medium supplemented with kinetin at different concentrations showed the highest total chlorophyll content (K2), highest total carotenoid content (K3) and better induction of lateral buds (K1). Hence, considering the promising response of artificial seawater supplemented with cytokinins and auxin for in vitro culture of the species, the present investigation could serve as a base study for formulating future research programmes.


Keywords: Rhodophyta, Seaweed, Media, PGR, Explants.
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How to cite this article:

Anuraj, A., Ajit Arun Waman, Chandra Prakash, S. Dam Roy, M. Viji, Manoj Baidya and Chadha, N.K. 2017. Investigating the Effects of Exogenous Factors on Growth, Photosynthetic Pigments and Bud Induction in Gracilaria corticata var. cylindrica under In vitro ConditionsInt.J.Curr.Microbiol.App.Sci. 6(9): 3235-3246. doi: https://doi.org/10.20546/ijcmas.2017.609.398