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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3073-3078
DOI: https://doi.org/10.20546/ijcmas.2017.609.378


A Proteomic Approach to Identify Seminal Plasma Proteins in Ostrich (Struthio camelus)
C. Pandian1*, S.T. Selvan2, M. Parthiban3 and S.M.K. Karthickeyan4
1Department of Poultry Science, MVC, TANUVAS, Chennai-07, Tamil Nadu, India
2PGRIAS, TANUVAS, Kattupakkam 603 203, Tamil Nadu, India
3Department of Animal Biotechnology, MVC, TANUVAS, Chennai-07, Tamil Nadu, India
4Dept. of Animal Genetics and Breeding, MVC, TANUVAS, Chennai-07, Tamil Nadu, India
*Corresponding author
Abstract:

The protein bands in ostrich seminal plasma (OSP) were identified by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and compared with NCBI reference sequence. The results revealed that OSP had five major specific proteins bands namely, OSP-I (GATA Zinc finger domain containing protein 1), OSP-II (GATA Zinc finger domain containing protein 1), OSP- III (E3 ubiquitin protein ligase RNF 216, OSP-IV (Mitotic spindle assembly checkpoint protein MAD1) and OSP-V (Dual specificity phosphatase DUPD1). The estimated molecular weight of OSP-I (Mr94.51 to 102.34 kDa), OSP-II (Mr75.19 to 93.07 kDa), OSP-III (Mr59.58 to 72.76 kDa), OSP-IV (Mr30.71 to 40.90 kDa) and OSP-V (Mr21.66 to 26.26 kDa). The results of our study provide basic knowledge of the protein composition of ostrich seminal plasma highlighting important physiological pathways which may play crucial roles in the sperm environment after ejaculation. This knowledge can be the basis to further develop procedures improving the reproduction of farmed ostrich.


Keywords: Proterin bands, ostrich seminal plasma (OSP)
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How to cite this article:

Pandian, C., Selvan, S.T., Parthiban, M. and Karthickeyan, S.M.K. 2017. A Proteomic Approach to Identify Seminal Plasma Proteins in Ostrich (Struthio camelus).Int.J.Curr.Microbiol.App.Sci. 6(9): 3073-3078. doi: https://doi.org/10.20546/ijcmas.2017.609.378