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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 3066-3072
DOI: https://doi.org/10.20546/ijcmas.2017.609.377


A Serological Study on Incidence of Brucellosis in Sheep in Ludhiana District of Punjab State of India
Neharika Saxena*, B.B. Singh, J.P.S. Gill and R.S. Aulakh
School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, India
*Corresponding author
Abstract:

We investigated the prevalence of Brucellosis in sheep in Ludhiana district of Punjab state of India. Serum samples of sheep (n=171) from slaughter houses and an organized farm were analyzed with common sero diagnostic tests RBPT, STAT, MAT and ELISA. Out of 171 sera, 31 were positive by one or more of the four tests; 12 were positive and 140 were negative by all the four tests. The prevalence was 10.52% by RBPT and ELISA. The mean anti-Brucella antibody titers were 41.53+18.69 by STAT and 52.30+32.03 by MAT. ELISA gave maximum true positives and RBPT gave minimum of false negative results. RBPT showed the highest sensitivity (78.26%) followed by ELISA (75%), STAT (68%) and MAT (50%), respectively. STAT had the highest specificity (98.63%) followed by ELISA (96.79%), RBPT and MAT (95.89% each), respectively. STAT had the highest positive predictive value of 89.47% followed by ELISA (79.26%), RBPT (75%) and MAT (66.67%), respectively. RBPT had the highest negative predictive value of 96.55% followed by ELISA (96.18%), STAT (94.74%) and MAT (92.11%), respectively. STAT had the highest positive likelihood ratio of 49.64 while MAT gave the highest negative likelihood ratio of 0.52.


Keywords: Brucellosis, Prevalence, Agglutination, Sheep, Sero- Epidemiology.
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How to cite this article:

Neharika Saxena, B.B. Singh, J.P.S. Gill and Aulakh, R.S. 2017. A Serological Study on Incidence of Brucellosis in Sheep in Ludhiana District of Punjab State of India.Int.J.Curr.Microbiol.App.Sci. 6(9): 3066-3072. doi: https://doi.org/10.20546/ijcmas.2017.609.377