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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 127-137
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.014


Screening of Potent Laccase Producing Organisms Based on the Oxidation Pattern of Different Phenolic Substrates
Sheena Devasia* and A. Jayakumaran Nair
Department of Biotechnology, University of Kerala, Kariavattom, Trivandrum, Kerala, India
*Corresponding author
Abstract:

Soil samples were collected from different forest areas of Kerala and screened for laccase activity using guaiacol plate assay technique. An increased concentration of guaiacol was used for the isolation of resistant strains. Substrate oxidation studies were carried out and the potent organisms were taken for enzyme production studies. The organisms, isolated by the screening strategies, were found efficient laccase producers. The potent organisms were identified as Arthrographis sp. and Enterobacter cloacae. The enzyme production rate of Arthrographis was found to increase logarithmically and a maximum quantity of 53 U/ml was obtained and the bacterial strain, Enterobacter cloacae, gave a maximum laccase activity of 8U/ml. The difference in the pattern of substrate oxidation by laccase from different organisms is paid attention in the study.


Keywords: Arthrographis, bacterial laccase, Enterobacter cloacae,guaiacol, laccase, lignin, oxidoreductase.
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How to cite this article:

Sheena Devasia and A. Jayakumaran Nair. 2016. Screening of Potent Laccase Producing Organisms Based on the Oxidation Pattern of Different Phenolic Substrates.Int.J.Curr.Microbiol.App.Sci. 5(5): 127-137. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.014