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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 109-119
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.012


Prevalence of Carbapenem Resistance and Comparison between Different Phenotypic Methods for Detection of Metallo-Β-Lactamases in Gram Negative Non-Fermentative Bacteria in the Acute Wards of a Tertiary Care Centre
N.K. Das1*, N. Grover2, R. Sriram2, Mahadevan Kumar2, V. L. Dudhat3 and S. Prasanna4
1Department of Microbiology, Dr. D. Y. Patil Medical College, Hospital and Research centre, Pimpri, Pune, India
2Department of Microbiology, Armed Forces Medical College, Pune, India
3Consultant Microbiologist, Sahyadri Hospital, Pune, India

4Department of Microbiology, ShriSathya Sai Medical College and Research Institute, Ammapettai, Kancheepuram, India
*Corresponding author
Abstract:

Non-fermentative Gram negative bacteria can cause life threatening infections in hospitalised patients. These organisms have high intrinsic antibiotic resistance. Carbapenems are potent agents against these. Production of carbapenemases especially MBLs can jeopardise its use. Study was done to detect the prevalence of carbapenem resistance in NFGNB and to compare the different phenotypic methods for detection of MBLs. Non-duplicate isolates were collected. Isolates were identified by using phenotypic methods and following standard laboratory protocols. Imipenem resistance was detected by using CLSI guidelines. A total of 296 isolates were collected. Acinetobacter baumannii was the predominant isolate (44.59%) followed by Pseudomonas aeruginosa.71.21% of A.baumannii and 52.06% of P.aeruginosa were imipenem resistant. 86.17% of A.baumannii and 82.53% of P.aeruginosa were modified Hodge test positive.67.02% of A.baumannii and 66.66% of P.aeruginosa were Double disk synergy test positive.76.59% of A.baumannii and 77.77% of P.aeruginosa were positive for combined disk test. 74.46% of A.baumannii and 76.19% of P.aeruginosa were positive for MBL by E-test. “Almost perfect agreement” was seen between CDT and E-test for A.baumannii whereas “Substantial agreement” was seen for P.aeruginosa. Hence CDT can be used as a cheaper method for phenotypic  detection of MBLs.


Keywords: MBL, carbapenemases, Non-fermenters, CDT, DDST, Pseudomonas aeruginosa, Acinetobacter baumannii.
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How to cite this article:

Das, N.K., N. Grover, R. Sriram, Mahadevan Kumar, V.L. Dudhat and Prasanna, S. 2016.
Prevalence of Carbapenem Resistance and Comparison between Different Phenotypic Methods for Detection of Metallo-Β-Lactamases in Gram Negative Non-Fermentative Bacteria in the Acute Wards of a Tertiary Care CentreInt.J.Curr.Microbiol.App.Sci. 5(5): 109-119. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.012