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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 2442-2446
DOI: https://doi.org/10.20546/ijcmas.2017.609.300


Screening Canine Microbiota for Extended Spectrum Beta-Lactamases (ESBLs) Using E-Test
N. Mohammad Sharif1*, B. Sreedevi1, R.K. Chaitanya1 and Ch. Srilatha2
1Department of Veterinary Microbiology
2Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India
*Corresponding author
Abstract:

Extended spectrum beta-lactamase (ESBL) production by animal pathogens is an emerging problem world-wide. E-test was performed for the detection of ESBL production in microbiota isolated from pyometra and otitis clinical cases of dogs presented to Veterinary clinics in Andhra Pradesh. Microbiota recovered from pyometra pus samples includes Escherichia coli (7, 46.6%), Staphylococcus (11, 73.3%) and Pseudomonas (12, 80%) species. Microbiota recovered from otitis pus samples includes Pseudomonas (8, 53.3%), Proteus vulgaris (9, 60%) and Staphylococcus (15, 100%) species. Of the seven E. coli isolates recovered, three (43%) isolates were positive for simultaneous production of ESBL and Amp C, two (28.5%) isolates were ESBL negative and two (28.5) isolates gave non-conclusive results. Of the 20 Pseudomonas isolates, 16 (80%) isolates were ESBL negative and four (20%) isolates gave non-conclusive results. All the 26 Staphylococcus and nine Proteus isolates were found negative for ESBL production.


Keywords: E. coli, ESBL, Otitis, Pseudomonas, Proteus, Pyometra, Staphylococcus.
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How to cite this article:

Mohammad Sharif, N., B. Sreedevi, R.K. Chaitanya and Srilatha, Ch. 2017. Screening Canine Microbiota for Extended Spectrum Beta-Lactamases (ESBLs) Using E-Test.Int.J.Curr.Microbiol.App.Sci. 6(9): 2442-2446. doi: https://doi.org/10.20546/ijcmas.2017.609.300