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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 2380-2385
DOI: https://doi.org/10.20546/ijcmas.2017.609.291


Screening and Partial Purification of Hydroxamate Type Siderophore from Pseudomonas sp.
Kuppuswamy Kavitha1, Arumugam Suganya1, Kuppusamy Umaa Rani2 and Balasubramaniyan Ramesh2*
1Department of Microbiology, Sri Sankara Arts and Science College, Enathur,Kanchipuram – 631561, Tamil Nadu, India
2Department of Biotechnology, Sri Sankara Arts and Science College, Enathur,Kanchipuram – 631561, Tamil Nadu, India
*Corresponding author
Abstract:

The present study document the isolation and enumeration of siderophore producing microbes from iron contaminated soil. Identification of potential strains and optimization studies for the production of siderophore was carried out. The Iron contaminated soil samples were collected from welding shops, factories in and around Kanchipuram and Thiruvallur. Ten morphologically different strains (AS1-AS10) were selected for further screening for the production of siderophores by Ferric Perchlorate Assay. Strain AS-6 showed a maximum OD value of 0.82 and was further identified as Pseudomonas sp and optimization studies were carried out. Siderophore production was maximum when using Glucose medium, 72 hours of incubation; pH 5.5, 300C; Ferric Chloride as additive. The partially purified siderophore showed good zone of inhibition against water borne pathogens such as Salmonella, Vibrio, Shigella, and E.coli.


Keywords: Siderophore, Pseudomonas, Waterborne pathogens.
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How to cite this article:

Kuppuswamy Kavitha, Arumugam Suganya, Kuppusamy Umaa Rani and Balasubramaniyan Ramesh. 2017. Screening and Partial Purification of Hydroxamate Type Siderophore from Pseudomonas spInt.J.Curr.Microbiol.App.Sci. 6(9): 2380-2385. doi: https://doi.org/10.20546/ijcmas.2017.609.291