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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(5): 45-53
DOI: http://dx.doi.org/10.20546/ijcmas.2016.505.005


Assessment of Biological Activities of Caffeine
Omkar Hemant Lele, Jinesh Anant Maniar*, Rohit Lalit Chakravorty, Shashikant Prabhakar Vaidya and Abhay Shadashiv Chowdhary
Clinical Pathology Department, Haffkine Institute for Training, Research and Testing,
Acharya Donde Marg, Parel, Mumbai 400012, India
*Corresponding author
Abstract:

Caffeine (1, 3, 7 – trimethylxanthine) is a member of methylxanthines, widely used as biological active substance in the world and known for its various pharmacological effects. This study investigated antimicrobial activity of caffeine and the ability of Subinhibitory Concentration’s (SIC’s) of the same to interfere with the bacterial proteolytic activity, hemolytic activity and biofilm formation. Synergistic activity of caffeine was also studied with currently used therapeutic drugs against MRSA. Antimicrobial activity against four clinical isolates of MRSA namely MRSA1, MRSA2, MRSA3, MRSA 4 along with standard strain Staphylococcus aureus ATCC 6538 by broth dilution method. Caffeine inhibited bacterial growth at 5 mg/mL concentration. Subinhibitory concentrations (SIC’s) thus selected were 1:2, 1:5 and 1:10 dilutions of MIC value i.e. 2.5 mg/ml, 1 mg/ml and 0.5 mg/ml respectively. There was a marked decrease in proteolytic activity in the range of 29% to 40%, hemolytic activity in the range of  9%- 19%) and in biofilm formation 14%- 21% in test organisms at 2.5 mg/ml. Marked synergistic activities were found against Mupirocin, Amoxyclav, Chloramphenicol, Rrifampicin, and Linezolid. Such activity of caffeine could be crucial for topical applications of it. Further characterization of caffeine with respect to its anti-cancer activity, immunomodulatory activity and mode of action need to be carried out to evaluate the biological activities and medicinal utility of caffeine.


Keywords: Caffeine, Minimum Inhibitory activity, Subinhibitory Concentration, Proteolytic activity,
Hemolytic activity
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How to cite this article:

Omkar Hemant Lele, Jinesh Anant Maniar, Rohit Lalit Chakravorty, Shashikant Prabhakar Vaidya and Abhay Shadashiv Chowdhary. 2016. Assessment of Biological Activities of CaffeineInt.J.Curr.Microbiol.App.Sci. 5(5): 45-53. doi: http://dx.doi.org/10.20546/ijcmas.2016.505.005