Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1883-1893
DOI: https://doi.org/10.20546/ijcmas.2017.609.232


Marker Assisted Gene Pyramiding of Leaf Rust Resistance Genes Lr24 and Lr28 in the Background of Wheat Variety DWR 162 (Triticum aestivum L.)
G. Anil Kumar1*, R.R. Hanchinal2, Shrinivas Desai1 and Suma Biradar3
1Department of Genetics and Plant Breeding, University of Agricultural Sciences,
Dharwad – 580005, Karnataka, India
2Chairperson, PPV and FR Authority, Ministry of Agriculture,Government of India, New Delhi, India
3MARS, University of Agricultural Sciences, Dharwad – 580005, Karnataka, India, India
*Corresponding author
Abstract:

Two highly effective genes for leaf rust resistance viz., Lr24, Lr28 were selected for pyramiding in the background of a susceptible but high yielding bread wheat variety DWR 162. The screening against most virulent pathotype of leaf rust 77-5 (121R63-1) indicated that both the genes confer a high degree of seedling resistance. The use of molecular markers, namely, SCS719, SCS1302607, SCS421570 and Xwmc313 validated the presence of resistance genes, Lr24 and Lr28 in DWR 162. The application of molecular markers facilitated identification of individual plants in BC2 -F1 and BC2 -F2 generations possessing the targeted genes. Finally 60 plants were selected in BC2 -F2 generation carrying the desired resistance genes, Lr24 and Lr28 in the background of DWR 162. The availability of combination of major rust resistance genes in desirable background would facilitate the strategic deployment of wheat varieties to achieve durable resistance.


Keywords: Triticum aestivum L., Pyramiding, Leaf rust, Resistance genes, Molecular markers.
Download this article as Download

How to cite this article:

Anil Kumar, G., R.R. Hanchinal, Shrinivas Desai and Suma Biradar. 2017. Marker Assisted Gene Pyramiding of Leaf Rust Resistance Genes Lr24 and Lr28 in the Background of Wheat Variety DWR 162 (Triticum aestivum L.).Int.J.Curr.Microbiol.App.Sci. 6(9): 1883-1893. doi: https://doi.org/10.20546/ijcmas.2017.609.232