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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1865-1873
DOI: https://doi.org/10.20546/ijcmas.2017.609.230


In Vitro Propagation of Cydonia oblonga cv. SKAU-016
Yusra Ali Basu, M.A. Mir, K.M. Bhatt and B.A. Mir
Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar – 191 121, India
*Corresponding author
Abstract:

Forced and unforced shoot tips were surface sterilized with different sterilant regimes and incubated under normal culture room conditions. Satisfactory culture asepsis (55.99%) and explant survival (56.66%) was achieved in forced explants by subjecting them to the treatment of 0.1 per cent Mercuric chloride for 10 minutes followed by 70 per cent ethyl alcohol for 10 seconds. Murashige and Skoog’s full strength medium with 0.50 mg /l BAP and 0.01 mg/l IBA gave the best response in terms of establishment of cultures. Murashige and Skoog’s full strength basal media supplemented with BAP and IBA at different concentrations were tried for culture proliferation of quince and it was found that BAP at 0.50 mg/l and IBA at 0.01 mg/l gave the highest per cent proliferated cultures (72.49 %), Shoot number/explants(5.75cm) and maximum shoot length(5.49cm). However, the lowest percentage of proliferated cultures (41.66%) were observed in WPM medium supplemented with BAP 0.50mg/l and IBA 0.01mg/l. Microcuttings (10-15mm) from the proliferated cultures were inoculated in two media supplemented with different IBA concentrations for rooting.MS medium fortified with IBA (1.25mg/l) resulted in maximum rooting (81.66%), root number/shoot (5.00%) and root length (6.15cm).


Keywords: Aseptic culture, in vitro propagation, in vitro rooting, Proliferation, Quince, Tissue culture.
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How to cite this article:

Yusra Ali Basu, M.A. Mir, K.M. Bhatt and Mir, B.A. 2017. In Vitro Propagation of Cydonia oblonga cv. SKAU-016.Int.J.Curr.Microbiol.App.Sci. 6(9): 1865-1873. doi: https://doi.org/10.20546/ijcmas.2017.609.230