National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was performed to assess the effect of diet supplementation with Nigella sativa (NS) and Sesame seeds (SS) on body weight, immune status, blood glucose (BG), insulin, total cholesterol (TC), triglycerides (TG) and kidney function parameters as well as on activities of renal tissue antioxidant enzymes in diabetic rats. Forty nine mature Sprague Dawley male rats were randomized into 7 groups (n= 7). Group (1) was used as a negative control and fed on basal diet, while the remaining 6 groups were rendered diabetic by intraperitoneal injection of alloxan (120 mg/kg). After induction of diabetes, group (2) was kept positive control, while groups (3), (4), (5), (6) and (7) were fed on diet supplemented with 5 % NS; 10% NS; 5% SS; 10% SS and 5% NS+ 5% SS, respectively for 6 weeks. The initial and final body weights of rats were recorded. Blood samples were collected for estimation of tumor necrosis factor alpha (TNF- ), inflammatory cytokines IL-4 and IL-8, fasting BG, insulin, TC and TG levels, blood urea nitrogen (BUN), uric acid and creatinine (Cr) serum levels. Differential leucocytic count and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in kidney tissues were also determined. The results showed that diet supplementation with NS and SS significantly increased body weight and improved feed efficiency ratio of diabetic rats. Diet supplementation with NS and SS decreased the number of lymphocytes, decreased production of TNF- and cytokines IL4 and IL8; normalized BG, insulin, TC and TG and BUN and Cr levels. There were enhanced the activity of SOD, GPx and CAT enzymes in renal tissues of diabetic rats. In conclusion, diet supplementation with NS and SS has immunostimulant, antidiabetic, hypolipidemic, nephroprotective and antioxidant activities in diabetic rats. Therefore, intake of baked foods and sweets supplemented with NS and SS may be beneficial for patients who suffer from diabetes mellitus.
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