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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 519-527
DOI: https://doi.org/10.20546/ijcmas.2017.609.062


Bacterial Isolation, Culture Sensitivity Test, Endometrial Cytology of Postpartum Cows and Assessment of Their Reproductive Performance
Akshay Sharma*, Madhumeet Singh, Pravesh Kumar, Amit Sharma, Amit Kashyap, Neelam, Indu Bala, Anurag Sharma, Nirdeshika Chaudhary and Pallavi Sharma
Dept. of Veterinary Gynecology and Obstetrics, College of Veterinary and Animal Sciences, Himachal Pradesh Agricultural University, Palampur – 176062, India
*Corresponding author
Abstract:

The objectives of this study were to find out the bacteria from postpartum dairy cows, determination of their antimicrobial sensitivity, to diagnose subclinical endometritis via endometrial cytology and evaluation of reproductive performance. Uterine discharge from normally parturited cows (n=23) were examined for aerobic bacterial identification and antimicrobial sensitivity at a weekly interval. Isolates include gram-positive bacteria (n=13), gram-negative bacteria (n=10) and mixed bacteria (n=7). The most frequently found isolates of bacteria were as follow: E. coli (32.26%), Bacillus cereus (22.58%), Staphylococcus aureus (16.13%), Bacillus cereus + Staphylococcus aureus (9.68%) and Escherichia coli + Proteus vulgaris (3.23%). From all 13 antimicrobial agents used in the culture sensitivity test, Levofloxacin and Ciprofloxacin were highly sensitive antibiotics and metronidazole was found resistant against both gram-positive and gram- negative bacteria. Endometrial cytology was performed in 18 cows, out of which 6 cows were diagnosed with subclinical endometritis. Reproductive performance of these cows was assessed via evaluation of days to first artificial insemination, number of inseminations per conception and calving to conception interval. In conclusion, this study provided an update on information of bacteria present in uterus after parturition, their status of antibiotic resistance and further effect on reproductive performance of cows.


Keywords: Bacterial isolation, Antimicrobial susceptibility, Endometrial cytology, Postpartum cows, Reproductive performance.
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How to cite this article:

Akshay Sharma, Madhumeet Singh, Pravesh Kumar, Amit Sharma, Amit Kashyap, Neelam, Indu Bala, Anurag Sharma, Nirdeshika Chaudhary and Pallavi Sharma. 2017. Bacterial Isolation, Culture Sensitivity Test, Endometrial Cytology of Postpartum Cows and Assessment of Their Reproductive Performance.Int.J.Curr.Microbiol.App.Sci. 6(9): 519-527. doi: https://doi.org/10.20546/ijcmas.2017.609.062