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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 356-367
DOI: https://doi.org/10.20546/ijcmas.2017.609.045


In silico Analysis to Identify Synteny in Bacterial Signatures of Iron Nanoparticles Synthesizing Bacteria Contributing to Big Data
Himani Sharma*, Ankita Sharma, Sashikant Sharma, Amna Devi and Poonam Shirkot
Department of Biotechnology, Dr. YS Parmar University of Horticulture and Forestry, Nauni, Solan (HP) 173 230, India
*Corresponding author
Abstract:

16S rrna gene sequencing technology is widely used to determine the microbial diversity which was earlier not possible with traditional methods. It is the most common housekeeping genetic marker which is used to study bacterial phylogeny and taxonomy. In this experiment iron nanoparticles synthesizing bacteria were isolated and characterized by morphological and biochemical test and confirmed by molecular approach using 16S rrna gene sequencing technology. The amplified gene sequence was compared to various bacterial genomes using various computational biology and bioinformatics tools in NCBI sequence database. Phylogenetic relationships and DNA sequencing identified three bacterial isolates as Stenotrophomonas maltophilia KBS 2.4, Bacillus cereus MSS 2.8 and Bacillus cereus MJS 3.0 of 1500bp after carrying BLASTn (NCBI Gene Bank Accession No: MF155656, MF155655, MF155656). Phylogenitic analysis and pairwise alignment revealed its close synteny with related species. A total of 100 bootstrap replications were performed to represent the evolutionary history of the taxa analysed. Using 16S rrna gene sequences, numerous bacterial genera and species have been classified and renamed, classification of uncultivable bacteria has been made possible, phylogenetic relationships have been determined, and the discovery and classification of novel bacterial species has been facilitated. Characterization of iron nanoparticles synthesizing bacteria would be helpful in defining wide variety of applications in near future. Further, in the last decade sequencing of various bacterial genomes and comparison between genomes has confirmed the representativeness of the 16S rrna gene in bacterial phylogeny.


Keywords: In silico, 16S rRNA sequencing, BLASTn, Microbial diversity, Evolutionary history.
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How to cite this article:

Himani Sharma, Ankita Sharma, Sashikant Sharma, Amna Devi and Poonam Shirkot. 2017. Insilico Analysis to Identify Synteny in Bacterial Signatures of Iron Nanoparticles Synthesizing Bacteria Contributing to Big Data.Int.J.Curr.Microbiol.App.Sci. 6(9): 356-367. doi: https://doi.org/10.20546/ijcmas.2017.609.045