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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 324-328
DOI: https://doi.org/10.20546/ijcmas.2017.609.041


Molecular Characterization of Enterotoxigenic Bacillus cereus Species Isolated from Meat Using Conventional PCR and Multiplex PCR
S. Fayaz1, G.A. Badroo2, Ajaz Ahmad3*, U. Rasool1, R. Mustafa1 and Mir Mudasir4
1Department of Food Technology, Doon Valley Institute of Engineering and Technology, Karnal, Haryana, India
2Department of Veterinary Microbiology, SKUAST-Jammu, India
3Department of Veterinary Pathology, GADVASU, Ludhiana, Punjab, India
4Department of Veterinary Pathology, SKUAST-Jammu, India
*Corresponding author
Abstract:

A total of 120 samples comprising 40 each of raw mutton, raw chicken and chicken biryani were analyzed for the enter toxigenic profile of Bacillus cereus. Out of 52 presumptive B. cereus isolates confirmed by conventional PCR method, 44 isolates produced a product of 475 base pairs on PCR by targeting species specific gyrB gene. Hence the incidence of B. cereus in meat and meat products was found to be 36.66 percent. Incidence of B. cereus in raw mutton, raw chicken and chicken biryani was 35 per cent, 30 per cent and 45 per cent. On subjecting to multiplex PCR, all the 44 isolates carried at least one of the six enterotoxin genes. One or more genes of HBL (hblCAD) and NHE (nheABC) complex were present in 28(63.63%) and 39(88.6%) isolates respectively. Based on the presence of one or more genes of hblCAD and nheABC complexes all the 44 isolates were further divided into six groups: G1 comprised of 20.45 per cent isolates which carried all the genes of both HBL and NHE complex while asG2 comprised of 31.81 per cent isolates which carried one or more genes of both HBL and NHE complex.. G3 comprised of 4.5per cent isolates which carried all the three genes of HBL while as G4comprised of 6.8 per cent isolates which carried one or more genes of HBL complex. G5 comprised of 18.18 per cent isolates which carried all the genes of NHE complex where as G6 comprised of 18.18 per cent isolates which carried one or more genes of NHE complex.


Keywords: Bacillus cereus, Meat, PCR, Enterotoxin, HBL, NHE
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How to cite this article:

Fayaz, S., G.A. Badroo, Ajaz Ahmad, U. Rasool, R. Mustafa and Mir Mudasir. 2017. Molecular Characterization of Enterotoxigenic Bacillus cereus Species Isolated from Meat Using Conventional PCR and Multiplex PCRInt.J.Curr.Microbiol.App.Sci. 6(9): 324-328. doi: https://doi.org/10.20546/ijcmas.2017.609.041