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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 255-279
DOI: https://doi.org/10.20546/ijcmas.2017.609.034


Algal Bioactive Metabolites against MDR Bacteria
R.A. Elnahas1, R. Salem1, R. Dorgham1, A. Elmorsy1, B. Elwakil3, S. Khattab2 and Z. Olama3*
1Industrial Microbiology and Applied Chemistry program
2Chemistry Department
3Microbiology Department, Faculty of Science, Alexandria University, Egypt
*Corresponding author
Abstract:

Seven algal species were collected from 7 different hotspots along Alexandrian Mediterranean shore; toluene and ethanol extract of U. fasciata and Enteromopha intestinalis showed the maximum antibacterial although Klebsiella pneumoniae (2) showed highest sensitivity towards the algal extracts while MRSA and MRSE were the most resistant organisms. MIC concentration was significantly low with Gram negative bacteria less than Gram positive bacteria. Time –Kill curve analysis showed that MRSA was less susceptible than Ps. aeruginosa with the same extract. SEM analysis of the samples showed aggregation and shrinkage in addition to leakage of the cells. LC50 was determined during the period of one week and no mortality was observed. Combination between algal extract and amikacin showed significant increase in the inhibition zone. Synergistic action was noticed between the silver nanoparticles (green synthesized by the U. fasciata extract) + amikacin (as known antibiotic) and U. fasciata extract (as the natural product). This synergy means less toxicity, less side effects and less cost. GC-MS-MS analysis of the U. fasciata sample showed Acyclic and noracyclic sesterterpenoid, pentacyclic triterpenoid, esters and carotenoid. Column chromatography was performed followed by antibacterial assay for the collected fractions to determine the active fraction. The isolated active compound was olean-12en-3, 15, 16, 21, 22, 28-hexol, with probability only 91.17% so probably our isolated compound is a derivative of oleanolic acid.


Keywords: U. fasciata, Antibacterial agent, Silver nanoparticles and New drug.
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How to cite this article:

Elnahas, R.A., R. Salem, R. Dorgham, A. Elmorsy, B. Elwakil, S. Khattab and Olama, Z. 2017. Algal Bioactive Metabolites against MDR BacteriaInt.J.Curr.Microbiol.App.Sci. 6(9): 255-279. doi: https://doi.org/10.20546/ijcmas.2017.609.034