Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1391-1402
DOI: https://doi.org/10.20546/ijcmas.2017.609.169


Immune Gene Expression against Similar Damselfish Virus (SRDV) Induced by Immunization with Inactivated Vaccine in Juvenile Koi Carp
P. Sivasankar*, K. Riji John, M. Rosalind George, M. Mohamed Mansoor, P. Magesh Kumar and M. Selvamagheswaran
Department of Fish Pathology and Health Management, School of Aquaculture, Fisheries College and Research Institute, Tamil Nadu Fisheries University, Thoothukudi - 628 008, Tamil Nadu, India
*Corresponding author
Abstract:

Vaccination is one of the best preventive measures against viral diseases in aquaculture. In this study, experimental inactivated similar damselfish virus (SRDV) vaccine was prepared and immunogenicity and protection against virus infection of the vaccine was investigated in koi carp (Cyprinus carpio). Formalin-inactivated virus at 4°C for 2 days was exhibited no typical clinical symptoms of SRDV infection and no mortality following challenge at 28-day post-vaccination. The immune response was induced by intraperitoneal injection of koi with formalin-inactivated vaccine added Quil-A® adjuvant. The expression levels of genes including IRF-7 and IL-10 in spleen of koi immunized with vaccine added Quil-A® adjuvant was highly up-regulated (52.2 fold) at 24 h post infection of the virus but in kidney was showed down-regulation (0.4 fold). Interestingly, the highest level of up-regulation (16 fold) was recorded at 96 h post infection of SRDV. Like IRF-7, highest up-regulation of IL-10 gene was observed in spleen of koi immunized with vaccine added Quil-A® adjuvant at 24 post infection of the virus while kidney was showed down-regulation. The study provides strong evidence for the existence of expression profiles of immune related genes (IRF-7 and IL-10) during the ranavirus infection. It also suggests that Quil-A® adjuvant enhances the immune response of the vaccine candidates.


Keywords: SRDV, Formalin-inactivated vaccine, Immune genes, Expression profiles
Download this article as Download

How to cite this article:

Sivasankar, P., K. Riji John, M. Rosalind George, M. Mohamed Mansoor, P. Magesh Kumar and Selvamagheswaran, M. 2017. Immune Gene Expression against Similar Damselfish Virus (SRDV) Induced by Immunization with Inactivated Vaccine in Juvenile Koi CarpInt.J.Curr.Microbiol.App.Sci. 6(9): 1391-1402. doi: https://doi.org/10.20546/ijcmas.2017.609.169