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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1282-1291
DOI: https://doi.org/10.20546/ijcmas.2017.609.154


Molecular Studies Ascertaining the Phylogenetic Relationships in Pomegranate (Punica granatum L.) Cultivars Using RAPD Markers
Komal Kathuria*, R. Bhargava, P.K. Yadav and Karun Gurjar
Department of Horticulture, College of Agriculture, SKRAU, Bikaner, Rajasthan, India
*Corresponding author
Abstract:

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and phylogenetic relationship among ten pomegranate cultivars. 14 decamer primers of OPF series were selected, produced a total of 114 bands; some species or cultivar specific bands were also obtained using RAPD markers. The number of amplified DNA bands varied between 3 (primer OPF-12) and 11 (primer OPF 3 and OPF 6). The size of the amplified band ranged between 300 and 6000 base pairs. These bands could serve as a useful aid for the varietal identification as well as are helpful for ascertaining the phylogenetic relationship among the cultivars studied. Genetic similarity was assessed using the Jaccard similarity coefficient. UPGMA cluster analysis revealed the dendrogram clearly separating cultivars Bhagwa and Phule Arakta from rest of the cultivars at a similarity coefficient of 0.85. The highest genetic similarity value coefficient was observed between genotypes Jalore seedless and Ganesh was 0.96. Cultivars G-137 and Phule Arakta were found to be genetically most diverse.


Keywords: RAPD, Phylogenetic relationship, Decamer primers, Cultivar specific bands, Jaccard similarity coefficient, UPGMA, Cluster analysis, Dendrogram.
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How to cite this article:

Komal Kathuria, R. Bhargava, P.K. Yadav and Karun Gurjar. 2017. Molecular Studies Ascertaining the Phylogenetic Relationships in Pomegranate (Punica granatum L.) Cultivars Using RAPD Markers.Int.J.Curr.Microbiol.App.Sci. 6(9): 1282-1291. doi: https://doi.org/10.20546/ijcmas.2017.609.154