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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1255-1265
DOI: https://doi.org/10.20546/ijcmas.2017.609.151


Isolation and Characterisation of the Indigenous Acetic Acid Bacteria from Western Ghats Soil Samples
Anurag Bellankimath, Ajay Katti, V.B. Hemalata* and Bharati S. Meti*
Department of Biotechnology, Basaveshwar Engineering College, Bagalkot, Karnataka, India
*Corresponding author
Abstract:

Acetic acid bacteria (AAB) are industrially important microorganism which is used in the production of alcohol and acetic acid. The study is carried out to Isolate and Characterise the bacteria isolated form different types of soil samples obtained from Western Ghats region. Various soil samples were collected from the Western Ghats of Belgaum and Khanapur region. These samples were screened for the presence of the acetic acid bacteria on the GYC media (glucose, yeast extract and calcium carbonate). The positive isolates were further grown in Carr medium supplemented with bromocresol blue to differentiate between the acetobacter and gluconobacter species. The biochemical tests were performed for characterization followed by 16s rRNA and the restriction analysis of the isolates. Out of the total twenty isolates of the acetobacter and gluconobacter, two acetobacter isolates were considered for 16s rRNA analysis based on the colony morphology and biochemical characterisation. The analysis revealed the presence of novel organisms which were renamed as Acetobacter pasteurianus strain YVB24 and Acetobacter pasteurianus strain AJN29, after characterisation of the strains they were deposited in Gene bank with the following accession numbers “KX831391” and “KX831390” respectively. The results suggest the positive isolation of indigenous acidic acid bacteria from the Western Ghats soil sample. This study aims to isolate the acetic acid bacteria found in the Western Ghats Soil and Characterise and study them for the production of the acetic acid. Hence it can be used for future studies.


Keywords: Acetobacter, Isolation, Soil, Western Ghats, GYC.
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How to cite this article:

Anurag Bellankimath, Ajay Katti, V.B. Hemalata and Bharati S. Meti. 2017. Isolation and Characterisation of the Indigenous Acetic Acid Bacteria from Western Ghats Soil Samples.Int.J.Curr.Microbiol.App.Sci. 6(9): 1255-1265. doi: https://doi.org/10.20546/ijcmas.2017.609.151