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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1103-1111
DOI: https://doi.org/10.20546/ijcmas.2017.609.132


Study of Acinetobacter Species with Special Reference to Species Differentiation and Metallo-Beta Lactamase Production in a Rural Tertiary Care Hospital
Kulkarni Vishal, Kinikar Anagha*, Bhalerao Deepika, Deorukhkar Sachin and Roushani Shahriar
Department of Microbiology, Rural Medical College, Pravara Institute of Medical Sciences (DU), Loni, Dist- Ahmednagar, Maharashtra, India
*Corresponding author
Abstract:

Acinetobacter has developed resistance against a number of broad spectrum drugs and is considered as multi-drug resistant bacteria. Metallo-beta-lactamase (MBL) production is important mechanisms of the resistance as they can hydrolyse almost all beta-lactam antimicrobial agents. To isolate Acinetobacter species from different clinical specimens and to determine their antimicrobial susceptibility pattern as well as to know the rate of MBL production. The study was done in Department of Microbiology, RMC, Loni. The clinical samples were subjected to Gram staining, aerobic culture using MacConkey and blood agar. Acinetobacter species were identified by standard microbiological identification methods. Antimicrobial susceptibility testing was done by Kirby Bauer disc diffusion technique and according to CLSI guidelines. The Imepenem resistant strains were subjected to MBL detection by Disk Potentiation test (DPT) and Epsilometer test. A total of 101(2.18%) Acinetobacter species were isolated out of 4627 clinical samples. Rate of isolation was maximum from burn department (38) followed by ICUs (23). A. baumanii (71) was the commonest species isolated followed by A. lowfii (18). The isolates were highly resistant to cefotaxime (100%), Co-trimoxazole (87%), Ciprofloxacin (78%), Cefepime (77%), Gentamicin (73%). All isolates were sensitive to Colistin. The number of MBL producing strain by DPT and E-test were 42 & 40 respectively. MBL production was detected better by DPT than E-test, therefore can be used as better screening method. Emergence of MBL producers and multidrug resistance in hospitals is alarming and reflect excessive use of carbapenems. Therefore early detection and prompt infection control measures are needed.


Keywords: Acinetobacter, Metallo-beta-lacatamase, Carbapenemase resistance.
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How to cite this article:

Kulkarni Vishal, Kinikar Anagha, Bhalerao Deepika, Deorukhkar Sachin and Roushani Shahriar. 2017. Study of Acinetobacter Species with Special Reference to Species Differentiation and Metallo-Beta Lactamase Production in a Rural Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(9): 1103-1111. doi: https://doi.org/10.20546/ijcmas.2017.609.132