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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(9): 1098-1102
DOI: https://doi.org/10.20546/ijcmas.2017.609.131


Detection and Characterization of Infectious Bronchitis Virus in Desi Birds by Molecular Assay
A.P. Surendar*, K.M. Palanivel, S. Saravanan and R. Gopala Krishnamurthy
Department of veterinary preventive medicine, Veterinary College and Research Institute, Namakkal-2, Tamil Nadu Veterinary and Animal Sciences University, India
*Corresponding author
Abstract:

Infectious Bronchitis (IB) is highly contagious and acute, with economically importance disease of poultry. 185 samples including tracheal swab and faecal swab were collected from apparently healthy and infected birds with respiratory disorders. An attempt made to isolate the virus through inoculation of specific pathogen free embryonated egg (SPFEE) as cultural medium. The embryo showing dwarfism were identified and subjected to molecular diagnosis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify N-Protein and sequenced. Factors responsible for prevalence of IB in desi chicken were studied. Out of 185 samples 7 samples showed dwarfism in SPFEE. 4 out of 7 samples, found positive for IBV by RT-PCR.


Keywords: Infectious Bronchitis, Isolation, Detection, RT-PCR, NP glycoprotein.
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How to cite this article:

Surendar, A.P., K.M. Palanivel, S. Saravanan and Gopala Krishnamurthy, R. 2017. Detection and Characterization of Infectious Bronchitis Virus in Desi Birds by Molecular Assay.Int.J.Curr.Microbiol.App.Sci. 6(9): 1098-1102. doi: https://doi.org/10.20546/ijcmas.2017.609.131